基于多孔硅的新型无标记结核病抗原抗体检测平台设计

发布时间：2018-01-13 12:00

本文关键词：基于多孔硅的新型无标记结核病抗原抗体检测平台设计　出处：《上海交通大学》2013年硕士论文　论文类型：学位论文

【摘要】：本课题旨在开发出一种快速、准确且成本低廉的结核病检测工具,以缓解结核病对人类健康的威胁。在结核病诊断方法中,血清学检测是一种相对低成本和快速的检测方法。目前,血清学检测中的两个主要方法——微球悬浮检测和酶联法检测都是标记检测。开发一种无标记的血清学检测工具,将能够再次提高血清学检测的效率。与此同时,多孔硅材料在生物传感中的应用变的越来越受人瞩目。多孔硅具有大表面积比,表面化学灵活,光学参数可调,制备成本低等优点。随着对多孔硅材料研究的深入,将多孔硅传感器应用于实际的生物检测已经成为可能。本文将血清学检测与多孔硅结合,设计并制备出一种可用于生物大分子检测的多孔硅无标记抗原抗体检测平台。然后,运用该传感器对结核病诊断中常用的16 kDa抗原及相应的抗16 kDa抗体进行检测试验,得出传感器的灵敏度及特异性等数据。通过一系列的实验验证,该传感器具有很高的特异性以及良好的灵敏度。之后,根据实验结果,本文提出了一系列的传感器优化方案,这些优化方案在仿真实验中得到证实。本文中所设计的传感器灵敏度最高可达0.05nm/μg/mL,最低检测限为1μg/mL。
[Abstract]:The purpose of this paper is to develop a rapid, accurate and low-cost tool for tuberculosis detection to alleviate the threat to human health caused by tuberculosis. Serological detection is a relatively low-cost and rapid detection method. The two main methods in serological detection, microsphere suspension detection and enzyme linked detection, are both marker detection. A non-labeled serological detection tool is developed. At the same time, the application of porous silicon in biosensor becomes more and more attractive. Porous silicon has large surface area ratio, flexible surface chemistry and adjustable optical parameters. With the development of porous silicon materials, it is possible to apply porous silicon sensors to practical biological detection. In this paper, serological detection is combined with porous silicon. To design and prepare a platform for the detection of non-labeled antigen antibodies of porous silicon for the detection of biological macromolecules. The 16 kDa antigen and the anti 16 kDa antibody commonly used in the diagnosis of tuberculosis were detected by this sensor. The sensitivity and specificity of the sensor are obtained. Through a series of experiments, the sensor has high specificity and good sensitivity. Then, according to the experimental results. In this paper, a series of sensor optimization schemes are proposed, which are verified by simulation experiments. The sensitivity of the sensor designed in this paper is up to 0.05 nm / 渭 g / mL. The lowest detection limit was 1 渭 g / mL.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：TP212;R52

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