一种条带免疫印迹试剂用于HIV抗体确证的效果评价

发布时间：2018-01-13 15:20

本文关键词：一种条带免疫印迹试剂用于HIV抗体确证的效果评价　出处：《中国艾滋病性病》2017年01期 　论文类型：期刊论文

【摘要】：目的评估一种条带免疫印迹试剂确证艾滋病病毒(HIV)抗体的效果。方法用蛋白印迹试验(WB)和条带免疫印迹试剂(LIA)平行检测1 035份标本,按照各自的说明书判断结果,对检测结果进行比较和分析。结果总体上,两种试剂检测的一致率为95.3%,对经过确证的640份HIV抗体阳性标本(含2份HIV-1O组和2份HIV-2标本)的检测的结果完全一致。在339份WB试剂判断为阴性的标本中,LIA试剂有5份(1.5%)为不确定结果,而在362份LIA试剂判断为阴性的标本中,WB试剂有28份(7.7%)为不确定结果(χ~2=15.29,P0.001)。在初次检测的341份标本中,确证了17份为HIV抗体阳性,考核试剂检测5份为不确定,12份为阳性。结论 LIA试剂显示出非特异反应暨不确定结果少的优势,可考虑用于HIV抗体的常规确证,并在实际应用中不断改进完善。
[Abstract]:Objective to evaluate the efficacy of a strip immunoblot reagent for the identification of HIV / AIDS antibody. Methods Western blot assay (WB) and strip Western blot reagent (LIA) were used to identify the HIV antibody. 1 035 specimens were detected in parallel. The results were compared and analyzed according to the results of their instructions. The results showed that the detection consistency rate of the two reagents was 95.3%. 640confirmed HIV antibody positive specimens (including 2 HIV-1O and 2 HIV-2). In 339 samples identified as negative by WB reagent. The results were uncertain in 5 samples of LIA reagent, and in 362 samples with negative LIA reagent. Twenty-eight samples of WB reagent were confirmed to be positive for HIV antibody (蠂 ~ (2 +)) (蠂 ~ (215.29) P 0.001). 17 of 341samples were confirmed to be positive for HIV antibody. Conclusion the LIA reagent shows the advantage of non-specific reaction and less uncertainty, so it can be considered for routine confirmation of HIV antibody. And in the practical application of continuous improvement and improvement.
【作者单位】：军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室;四川省疾病预防控制中心;河南省疾病预防控制中心;
【分类号】：R512.91
【正文快照】： 2.四川省疾病预防控制中心,成都610000;3.河南省疾病预防控制中心,郑州450016)Corresponding author:Bao Zuoyi,Email:bzy_6611@sina.com艾滋病病毒(HIV)感染引起的艾滋病(AIDS),是全球重大公共卫生问题之一。迄今为止,全球已有3 400万人感染HIV;2014年,全球因AIDS死亡人数为1

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