核酸检测HBV DNA阳性献血者的追踪检测结果分析

发布时间：2018-01-14 20:10

本文关键词：核酸检测HBV DNA阳性献血者的追踪检测结果分析　出处：《中国输血杂志》2017年05期 　论文类型：期刊论文

【摘要】：目的调查ELISA检测合格献血者的核酸检测结果,并对NAT阳性献血者追踪检测,为保证临床输血安全提供依据。方法经2种ELISA试剂检测合格的献血者血液标本,采用上海浩源核酸检测系统进行8人份混样检测,混检反应性的标本进行拆分,以拆分结果为最终报告结果,并对NAT阳性献血者追踪检测。结果 1)采集的40 496份献血者标本中,ELISA检测合格标本40 189份,不合格标本307份。2)ELISA检测合格的40 189份标本中共检出HBV DNA阳性22例,未检出HCV RNA、HIV-1 RNA阳性标本。3)已完成17例HBV DNA阳性献血者的追踪检测,其中14例为隐匿性HBV感染,未发现"窗口期"感染的献血者。结论 1)2遍ELISA检测后仍存在输血感染风险,增加NAT检测可进一步提高临床输血安全水平。2)追踪调查显示,HBV DNA阳性献血者中以OBI为主,为输血残余风险的主要原因。
[Abstract]:Objective to investigate the results of nucleic acid detection ELISA detection of qualified blood donors, and NAT positive blood donors tracking, to provide a basis to ensure the safety of clinical transfusion. Methods after 2 ELISA reagents tested blood samples of blood donors, 8 were mixed sample detected by Shanghai grandsoo nucleic acid detection system, detection of mixed reaction were split the results of the final report, in order to split the results, and NAT positive blood donors detected. Results 1) collected 40496 samples of blood donors were detected, ELISA qualified specimens of 40189 copies, 307 copies of specimen failure).2 ELISA detection of 40189 samples qualified detected HBV DNA positive 22 cases, HCV was not detected in RNA HIV-1, RNA.3) positive samples were detected in 17 cases of HBV has been tracking DNA positive blood donors, including 14 cases of occult HBV infection was not found in the "window period" of blood donors infected. Conclusion: 1) there is still 2 times the ELISA detection after blood transfusion Infection risk and increased NAT detection can further improve the safety level of clinical transfusion..2) follow up survey shows that HBV DNA positive blood donors are mainly OBI, which is the main reason for residual risk of blood transfusion.

【作者单位】：焦作市中心血站;
【分类号】：R512.62
【正文快照】： 核酸扩增检测技术(NAT)具有高度的敏感性和特异性,检测均合格的献血者核酸标本进行8人份混样检测,每批检可检测出标本中极微量的病毒核酸,显著缩短病毒的“窗口测均设置阴、阳性对照及室内质控各1个pool,混检结果无反期”[1],发现血清学检测无法检出的潜在感染,从而减少输血应

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