丙肝病毒核心抗原与丙肝抗体联合检测在丙型肝炎诊断中的应用

发布时间：2018-01-15 22:23

本文关键词：丙肝病毒核心抗原与丙肝抗体联合检测在丙型肝炎诊断中的应用　出处：《实用医学杂志》2015年09期 　论文类型：期刊论文

【摘要】：目的:通过检测丙型肝炎病毒RNA(HCV-RNA)、丙型肝炎病毒核心抗原(HCV-c Ag)、丙型肝炎抗体(HCV-Ab),探讨联合检测在丙肝诊断中的价值。方法:用ELISA方法对3 000例门诊及住院患者进行HCV-c Ag与HCV-Ab的联合检测,对单项阳性或两项均阳性者同时进行PCR法HCV-RNA检测。结果:3 000例患者中,进行HCV-c Ag与HCV-Ab联合检测,单项阳性或两项均阳性共45例,以HCV-RNA阳性作为分组标准,经HCV-RNA确证阳性39例,阴性6例,HCV-c Ag检测的阳性预测值为94.1%,HCV-Ab检测的阳性检出率为94.9%,两种方法联合检测可大大提高HCV临床检出率。结论 :HCV-c Ag检测与HCV-Ab检测能相互补充,两者联合检测更理想,能有效减少丙肝窗口期的漏检,有助于实现丙肝的早期防治,值得临床推广应用。
[Abstract]:Objective: through the detection of hepatitis C virus RNA (HCV-RNA), hepatitis C virus core antigen (HCV-c Ag), hepatitis C antibody (HCV-Ab), to investigate the combined detection of hepatitis C in the diagnostic value. Methods: 3000 cases of outpatient and combined detection of HCV-c Ag and HCV-Ab in patients with ELISA method and PCR method. HCV-RNA detection of single positive or two were positive. Results: in 3000 cases, combined detection of HCV-c and HCV-Ab were Ag, two were positive or positive in 45 cases, HCV-RNA positive as grouping criteria by HCV-RNA proves positive in 39 cases, 6 cases were negative, the positive predictive value for the detection of HCV-c Ag 94.1%, the positive rate of HCV-Ab detection was 94.9%, the two combined detection method can greatly improve the detection rate of clinical HCV. Conclusion: HCV-c Ag detection and HCV-Ab detection can complement each other, the combined detection of hepatitis C is more ideal, can effectively reduce the window period It is helpful to realize the early prevention and cure of hepatitis C, which is worthy of clinical application.

【作者单位】：广东省肇庆市第二人民医院;
【分类号】：R512.63;R446.6
【正文快照】： 丙型肝炎是由丙型肝炎病毒(HCV)感染引起的重要的世界性传染病之一,在中国人群的感染率达3.2%,多数HCV感染都是无症状的,发病比较隐蔽,极容易转为慢性,且有相当比例患者会发展为肝硬化、肝癌[1],对人类健康危害十分严重。HCV是单股正链RNA病毒,主要通过输血、注射、密切接触等

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