结核潜伏感染蛋白Rv2657c T细胞和B细胞表位的预测与分析

发布时间：2018-01-20 16:10

本文关键词： 结核病 Rvc 表位预测　出处：《实用医学杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的:预测结核分枝杆菌Rv2657c蛋白的抗原表位,了解其免疫原性。方法:应用DNAStar软件预测Rv2657c蛋白的T细胞和B细胞表位;Blast方法分析该蛋白氨基酸序列与人类蛋白序列的相似性;SYFPEITHI超基序法、BIMAS量化基序法及Net CTL法预测蛋白的CTL表位;RANKPEP及SYFPEITHI超基序法远程预测Th细胞表位。结果:DNAStar软件预测Rv2657c蛋白有5个B细胞抗原表位,6个T细胞抗原表位。SYFPEITHI超基序法、BIMAS量化基序法及Net CTL法预测该蛋白有6个CTL表位;RANKPEP及SYFPEITHI超基序法预测该蛋白有38个Th表位。结论:Rv2657c蛋白既有B细胞表位也有T细胞表位,可能成为结核病诊断抗原分子和候选疫苗抗原。
[Abstract]:Objective: to predict the antigenic epitopes of Rv2657c protein of Mycobacterium tuberculosis and to understand its immunogenicity. Methods: DNAStar software was used to predict the T and B cell epitopes of Rv2657c protein. The amino acid sequence of the protein was similar to that of human protein by Blast. SYFPEITHI hypermoticons were used to predict the CTL epitopes of proteins by quantitative motifs of BIMAS and Net CTL. RANKPEP and SYFPEITHI hypermotifs were used to predict Th cell epitopes. Results 5 B cell epitopes of Rv2657c protein were predicted by the software of SYFPEITHI Star. Six T cell epitopes. SYFPEITHI were used to predict the existence of six CTL epitopes by BIMAS quantization motifs and Net CTL methods. RANKPEP and SYFPEITHI hypermotifs were used to predict 38 Th epitopes of the protein. Conclusion: 1 Rv2657c protein has both B cell epitopes and T cell epitopes. It may be a diagnostic antigen molecule and a candidate vaccine antigen for tuberculosis.
【作者单位】：河北北方学院;解放军第三0九医院全军结核病研究所/全军结核病防治重点实验室;
【基金】：“十二五”国家重大科技专项(编号:2013ZX10003003-005) 军队医学科技“十二五”重点项目(编号:BWS11J050) 北京市科技创新基地培育与发展工程专项(编号:Z141107004414021) 解放军第309医院重点课题(编号:2015ZD-004)
【分类号】：R52
【正文快照】： 结核病是全球第二大主要致死性传染病,据报道,2014年全球新发现600万结核病患者,150万人死于结核病[1]。全球大约有1/3的人口感染了结核分枝杆菌(mycobacterium tuberculosis,Mtb)[2]。Mtb感染后被机体抑制,但是又不能完全清除,发展为结核潜伏感染(latent tuberculosis infect

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