TLR2及其相关细胞因子在军团菌感染中作用

发布时间：2018-01-24 20:43

本文关键词： Toll样受体(TLR) 肿瘤坏死因子-α(TNF-α) 白介素β(IL-β) 白介素(IL-) 军团菌感染　出处：《中国公共卫生》2015年04期 　论文类型：期刊论文

【摘要】：目的通过体外实验探讨Toll样受体2(TLR2)及肿瘤坏死因子-α(TNF-α)、白介素1β(IL-1β)、白介素6(IL-6)在军团菌感染中的作用。方法分离30名健康人外周血单核细胞(PBMC),将每名健康人PBMC分为对照组和军团菌刺激组,军团菌刺激组加入500μL 2×107/m L军团菌悬液,对照组加入500μL磷酸盐缓冲液(PBS),分别在18、24、72 h时获取细胞及上清液,采用real-time PCR法测定细胞中TLR2、My D88 mRNA的表达水平,ELISA法检测细胞培养上清液IL-6、IL-1β及TNF-α含量。结果与对照组比较,18h军团菌刺激组My D88 mRNA△Ct值(1.414 0±0.044 9)低于对照组(1.684 4±0.042 6),24h军团菌刺激组TLR2 mRNA△Ct值(1.347 9±0.056 2)低于对照组(1.425 9±0.038 7),72h军团菌刺激组TLR2 mRNA和My D88 mRNA△Ct值[(1.504 9±0.018 9)和(1.540 0±0.031 9)]均高于对照组[(1.410 5±0.025 5)和(1.339 2±0.032 2)],差异均有统计学意义(均P0.05);18、24、72h军团菌刺激组细胞上清液中TNF-α、IL-1β、IL-6浓度均高于对照组,差异均有统计学意义(均P0.05)。结论 TLR2及TNF-α、IL-1β、IL-6等相关炎性因子可有效抵制军团菌感染,TLR2介导的天然免疫可能是军团菌感染的保护因素。
[Abstract]:Objective to investigate the expression of Toll like receptor 2 (TLR2) and tumor necrosis factor- 伪 (TNF- 伪), interleukin-1 尾 (IL-1 尾) in vitro. Methods 30 healthy individuals were isolated from peripheral blood monocytes and divided into control group and Legionella stimulation group. In the Legionella group, 500 渭 L 2 脳 10 7 / mL Legionella suspensions were added in the control group, and in the control group, 500 渭 L phosphate buffer (PBSN) was added in 1824, respectively. Cells and supernatants were obtained at 72 h. The expression of TLR2My D88 mRNA was measured by real-time PCR assay. The levels of IL-6 and TNF- 伪 in the supernatant of cell culture were determined by ELISA method, and the results were compared with those in the control group. The value of my D88 mRNA Ct in the 18 h Legionella stimulating group was lower than that in the control group (1.414 0 卤0.044 9), and was significantly lower than that in the control group (1.684 4 卤0.042 6). The TLR2 mRNA Ct value of the 24 h Legionella stimulation group was 1.347 9 卤0.056 2, which was lower than that of the control group (1.425 9 卤0.038 7). TLR2 mRNA and my D88 mRNA Ct in Legionella spp. [1.5049 卤0.018 9) and 1.5400 卤0.031 9) were higher than those in the control group. [The difference was statistically significant between 1.4105 卤0.025 5 and 1.339 2 卤0.032 2). The concentration of TNF- 伪, IL-1 尾 and IL-6 in the supernatant of Legionella spp. Stimulated by Legionella spp. 1824 h was higher than that in the control group. Conclusion TLR2, TNF- 伪, IL-1 尾 and IL-6 can effectively resist Legionella infection. TLR2 mediated innate immunity may be the protective factor of Legionella infection.
【作者单位】：山西医科大学公共卫生学院流行病学教研室;
【基金】：国家自然科学基金(30872154) 高等学校博士学科点专项科研基金(20040114004)
【分类号】：R517.9
【正文快照】： 军团菌病是1976年发现的以群体发热及非典型肺炎为基本特征的急性呼吸道传染病。嗜肺军团菌(Legionella pneumophila,LP)是引起非典型肺炎的重要病原菌[1]。Toll样受体(Toll like receptors,TLRs)可识别并结合不同细菌或病毒的分子结构,启动机体天然免疫清除病原微生物[2]。TL

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