嘉兴地区献血人群隐匿性乙肝病毒感染血清学及病毒学特征研究

发布时间：2018-01-26 08:19

  本文关键词： 隐匿性乙型肝炎病毒感染 HBsAg 抗-HBc 献血者 核酸扩增技术　出处：《中国输血杂志》2017年07期 　论文类型：期刊论文

【摘要】：目的研究分析嘉兴地区献血人群隐匿性乙肝病毒(OBI)感染血清学及病毒学特征。方法采用常规的ELISA(HBsAg、抗-HCV、抗-HIV、抗-TP)和核酸扩增技术(NAT)对本中心52 698份无偿献血者标本进行联合筛查,对NAT+标本做进一步鉴别检测病毒类型;收集HBs Ag-/HBV-DNA+的标本再另选3种不同的HBs Ag酶免试剂盒定性检测,并选用化学发光对HBsAg和抗-HBs定量检测,同时采用实时荧光PCR(QPCR)进行HBV核酸病毒载量测定;再结合血清学乙肝三系标志物、追踪检测和一般流行病统计学资料(性别、献血次数和年龄)来进一步分析研究OBI的血清学及病毒学相关分布情况。结果共确认47例OBI感染者,OBI流行率为0.89‰(1∶1 121),窗口期(WP)2例(1∶26 349);HBsAg、HBeAg检测结果均为阴性,发现6种OBI血清学模式,抗-HBs定量100 m IU/m L的标本占27.66%(13/47),抗-HBc+占91.49%(43/47),HBV-DNA核酸定量范围(4.10-1.82)×10~3(IU/m L)(中位数15.83),5例阳性对照HBsAg+/HBV-DNA+病毒载量范围(61.47-1.28)×104(IU/m L)(中位数538.15),两组结果比较,其差异有统计学意义(P0.05);40岁以上的男性献血者OBI感染率高(P0.05),多次献血者与首次献血者OBI感染率差异有统计学意义(0.01P0.05)。结论 OBI感染者病毒载量低,以抗-HBc+为主要血清学表现形式;NAT可以检出OBI,缩短窗口期,有利于保障临床血液安全。
[Abstract]:Objective to study the serological and virological characteristics of occult hepatitis B virus (OBI) infection among blood donors in Jiaxing. Methods routine Elisa HBsAg, anti-HCV and anti-HIV were used. Anti-TP-) and nucleic acid amplification technique (Nat) were used to screen 52 698 volunteer blood donor samples in our center, and NAT samples were further identified and tested for virus types. The samples of HBsAg-/-r HBV-DNA were collected and three different kinds of HBsAg enzyme immunoassay kits were selected for qualitative detection, and chemiluminescence was used to detect HBsAg and anti-HBs quantitatively. At the same time, the viral load of HBV nucleic acid was determined by real-time fluorescence PCR. Combined with serological hepatitis B three series markers, tracking detection and general epidemiological statistical data (gender). To further analyze the serological and virological distribution of OBI. Results 47 cases of OBI infection were confirmed. The prevalence rate of OBI was 0.89 鈥，

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