2011-2014年常州地区HIV-1抗体阳性者免疫印迹带型分析

发布时间：2018-01-30 02:01

本文关键词： HIV-抗体 WB WB带型　出处：《现代预防医学》2015年22期 　论文类型：期刊论文

【摘要】：目的分析2011-2014年常州地区HIV-1抗体阳性者的免疫印迹(Western Blot,WB)带型。方法应用WB试验对2011-2014年常州地区HIV-1抗体阳性待确证标本进行确证检测,并对其WB带型进行统计学分析。结果1 144份HIV-1抗体待确证标本中,36份确证为阴性,71份为不确定,1 037份为阳性,阳性率为90.7%。在HIV-1抗体阳性标本WB带型中,gp160阳性率最高,为96.5%;p24和gp120次之,阳性率均在90.0%以上;gp41、p66、p51和p31阳性率较低,均低于90.0%;p17、p55和p39阳性率分别为66.4%、45.7%和41.9%;WB带型阳性率男女性之间差异均无统计学意义(P0.05);20~39岁组的p39阳性率高于其他年龄组(χ2=10.861,P0.05),20岁组的p17阳性率高于其他年龄组(χ2=7.906,P0.05)。结论 p39和p17带型阳性率在4个年龄组之间的差异均有统计学意义;gp160、gp120、gp41和p24是HIV-1抗体确证阳性的重要条带,且本地区检测的HIV-1抗体阳性者多在AIDS期前。
[Abstract]:Objective to analyze the Western blot Blot of HIV-1 antibody positive patients in Changzhou area from 2011 to 2014. Methods WB test was used to detect the positive specimens of HIV-1 antibody in Changzhou from 2011 to 2014. The WB banding patterns were analyzed statistically. Results among the 1 144 HIV-1 antibody samples, 36 were confirmed as negative and 71 were uncertain and 1 037 were positive. The positive rate of gp160 in HIV-1 antibody positive samples was 90.7. The positive rate of gp160 was the highest (96.5b) in HIV-1 antibody positive samples. The positive rates of p24 and gp120 were above 90.0%; The positive rates of gp41, p66, p51 and p31 were lower than 90.0; The positive rates of p17, p55 and p39 were 45.7% and 41.9%, respectively. There was no significant difference between male and female in the positive rate of WB band type (P 0.05). The positive rate of p39 in the group of 20 ~ 39 years old was higher than that in the other age group (蠂 ~ 2 10.861) and the positive rate of p17 in the group of 20 years old was higher than that in the other age group (蠂 ~ 2 + 7.906). Conclusion the positive rates of p39 and p17 bands were significantly different among the four age groups. Gp160, gp120, gp41 and p24 were important bands for HIV-1 antibody confirmation, and most of the HIV-1 antibody positive in our area were before AIDS stage.
【作者单位】：常州市疾病预防控制中心检测检验中心;
【分类号】：R446.6;R512.91
【正文快照】： 我国自上世纪80年代发现第一例艾滋病病例至今,各地市艾滋病感染者呈逐年增长趋势。国内HIV抗体检测流程均为先初筛,初筛阳性反应标本经复检后,仍呈阳性反应再进行HIV抗体确证试验。免疫印迹试验(Western Blot,WB)因其高特异性和敏感性,是国内HIV抗体确证检测机构的首选方法[1

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