2010-2012年北京市部分地区MSM中HIV-1急性期感染者的毒株亚型和流行状况

发布时间：2018-02-02 14:35

本文关键词： 艾滋病病毒型男男性行为者基因亚型系统进化树　出处：《中国艾滋病性病》2017年05期 　论文类型：期刊论文

【摘要】：目的研究北京市男男性行为者(MSM)艾滋病病毒1型(HIV-1)感染者的毒株亚型、流行现状和变化规律。方法采用单纯随机抽样的方法,抽取北京佑安医院2010-2012年新发现的MSM HIV-1急性期感染者的血样共100人份,采样前均未进行抗病毒治疗。提取外周血单核细胞(PBMC)中的脱氧核糖核酸(DNA),用巢式聚合酶链式反应(PCR)扩增病毒env、gag和pol基因区序列,三区中如有扩增失败,则尝试提取血浆中的核糖核酸(RNA),反转录后进行巢式PCR扩增。PCR产物测序后进行基因亚型分析。计算pol基因区样本间基因离散率,并做系统进化树分析。结果有96份样本的基因序列扩增成功,检出的基因亚型有CRF01_AE、CRF07_BC、B、B′、CRF55_01B、CRF65_cpx和未知亚型,所占比例依次为42.7%、25.0%、15.6%、1.0%、1.0%、3.1%和11.5%。前3种亚型合计占83.3%。各亚型所占比例与相关文献相比有明显变化,表现为CRF07_BC亚型所占比例的快速上升和B亚型所占比例的显著下降。发现了新亚型CRF65_cpx、CRF55_01B和一些未知亚型。结论 2010-2012年,北京市MSM HIV-1急性期感染者的HIV-1毒株亚型分布与相关报道相比已经发生改变,建议对北京地区HIV-1毒株的流行和变异进行密切监测。
[Abstract]:Objective to study the subtype, prevalence and variation of HIV 1 (HIV-1) infection in MSM (MSM) patients in Beijing. Methods A simple random sampling method was used. A total of 100 blood samples were collected from newly discovered MSM HIV-1 patients in Beijing Youan Hospital from 2010 to 2012. DNA was extracted from peripheral blood mononuclear cells (PBMC) and env was amplified by nested polymerase chain reaction (PCR). Gag and pol gene sequences, if there is a failure of amplification in the three regions, try to extract RNA RNA from the plasma. After reverse transcription, nested PCR amplification. PCR products were sequenced and gene subtypes were analyzed. The gene dispersion rate among samples of pol gene region was calculated. The results of phylogenetic tree analysis showed that 96 samples were amplified successfully, and the gene subtypes detected were CRF01AECOF07BCnBC01-CRF5501B. The proportion of CRF65_cpx and unknown subtypes was 42.7% and 25.0%, and 15.6%, 1.0% and 1.0%, respectively. The first three subtypes accounted for 83.3% of the total. The proportion of each subtype was significantly different from that of the related literature. The results showed that the proportion of CRF07_BC subtype increased rapidly and that of B subtype decreased significantly. A new subtype CRF65_cpx was found. CRF55_01B and some unknown subtypes. Conclusion 2010-2012. The distribution of HIV-1 subtypes of MSM HIV-1 infected patients in Beijing has changed compared with the related reports. It is suggested to monitor closely the prevalence and variation of HIV-1 strains in Beijing.
【作者单位】：中国疾病预防控制中心性病艾滋病预防控制中心;首都医科大学附属北京佑安医院感染中心;
【基金】：北京市医院管理局临床医学发展专项经费(ZY201401) 北京市艾滋病研究重点实验室(BZ0089) 首都卫生发展科研专项基金项目(首发2016-1-2182) 北京市科技计划资助(D141100000314002,D141100000314005) 国家“十二五”科技重大专项(2012ZX10001001-001) 中美艾滋病防治合作项目(2016-2017,4.2)~~
【分类号】：R181.3;R512.91
【正文快照】： 男男性行为者(MSM)是艾滋病病毒(HIV)感染的高危人群之一。由于易发生非保护性性行为、多性伴侣以及商业性性行为等高危性行为,在HIV感染者中MSM所占比重逐年增加[1-2]。据中国疾病预防控制中心统计,我国新报告的HIV感染者和艾滋病病人中,MSM所占比例已从2006年的2.5%上升到20

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