尖锐湿疣患者疣体病变皮损组织中Fas、FasL及Foxp3表达与HPV DNA载量的相关性研究

发布时间：2018-02-02 17:21

本文关键词： 尖锐湿疣皮损组织人凋亡相关因子配体翼状螺旋转录因子P　出处：《中华医院感染学杂志》2017年06期 　论文类型：期刊论文

【摘要】：目的探讨尖锐湿疣患者皮损组织中人凋亡相关因子(Fas)、人凋亡相关因子配体(FasL)及翼状螺旋转录因子P3(Foxp3)表达与HPV DNA载量的相关性。方法选择2015年1月-12月在医院治疗的尖锐湿疣患者皮损组织100份为尖锐湿疣组,选择包皮环切术正常包皮组织50例为正常包皮组;HPV DNA采用PCR荧光检测,Fas、FasL及Foxp3采用免疫组化法检测;比较尖锐湿疣组织与正常包皮组织中Fas、FasL及Foxp3表达,比较不同HPV DNA载量尖锐湿疣组织中Fas、FasL及Foxp3表达,分析Fas、FasL及Foxp3表达与HPV DNA载量的相关性。结果尖锐湿疣组织中Fas阳性率显著低于正常包皮组(P0.05),而FasL阳性率显著高于正常包皮组(P0.05);HPV DNA高载量组Fas阳性率显著低于低载量组,而FasL显著高于低载量组(P0.05);尖锐湿疣组Foxp3阳性标记指数及阳性率显著高于正常包皮组(P0.05);HPV DNA高载量组Foxp3阳性标记指数及阳性率显著高于低载量组(P0.05);相关性分析结果显示:尖锐湿疣组织中Fas表达与HPV DNA载量呈显著负相关(r=-0.281);FasL与HPV DNA载量呈显著正相关(r=0.346);Foxp3与HPV DNA载量呈显著正相关(r=0.413)。结论尖锐湿疣组织中Fas表达下降,与HPV DNA呈负相关,FasL及Foxp3表达上升,与HPV DNA呈正相关。
[Abstract]:Objective to investigate the apoptosis-related factor FasA in lesions of patients with condyloma acuminatum. Human apoptosis-related factor ligand FasL and winglike helical transcription factor P3Foxp3). Methods 100 patients with condyloma acuminatum treated in hospital from January 2015 to December were selected as condyloma acuminatum group. 50 cases of normal prepuce tissue were selected as normal prepuce group. HPV DNA was detected by PCR fluorescence and Foxp3 by immunohistochemistry. To compare the expression of Fas-FasL and Foxp3 in condyloma acuminatum tissue and normal prepuce tissue, and the expression of Fas-FasL and Foxp3 in condyloma acuminatum tissue with different HPV DNA load. Results the positive rate of Fas in condyloma acuminatum tissue was significantly lower than that in normal prepuce group (P 0.05). The positive rate of FasL was significantly higher than that of normal prepuce group (P 0.05). The positive rate of Fas in HPV DNA high load group was significantly lower than that in low load group, while FasL was significantly higher than that in low load group (P 0.05). The positive labeling index and positive rate of Foxp3 in condyloma acuminatum group were significantly higher than those in normal prepuce group (P 0.05). The positive labeling index and positive rate of Foxp3 in HPV DNA group were significantly higher than those in low load group (P 0.05). The results of correlation analysis showed that the expression of Fas in condyloma acuminatum was negatively correlated with the HPV DNA load. There was a significant positive correlation between FasL and HPV DNA load. There was a significant positive correlation between Foxp3 and HPV DNA load. Conclusion the expression of Fas decreased in condyloma acuminatum and negatively correlated with HPV DNA. The expression of FasL and Foxp3 increased, which was positively correlated with HPV DNA.
【作者单位】：河南省南阳市中心医院皮肤科;
【基金】：河南省卫生计生委科技攻关基金资助项目(201406233)
【分类号】：R752.53
【正文快照】： 尖锐湿疣是临床常见的传染性疾病,由人类乳头瘤病毒感染导致,属于性传播疾病。目前HPV病毒可鉴别的亚型有100多种,而其中34种亚型与尖锐湿疣的发病密切相关,而其中6、11、16、18亚型最常见。16、18亚型属于高风险基因型,与生殖器肿瘤密切相关,易继发鳞癌[1]。FasL(人凋亡相关

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