HBV转基因小鼠体内淋巴细胞表型特点及干扰素α对其的影响

发布时间：2018-02-06 07:21

本文关键词： 乙型肝炎病毒转基因小鼠干扰素α 免疫调节　出处：《南方医科大学学报》2016年06期 　论文类型：期刊论文

【摘要】：目的探讨乙型肝炎病毒(HBV)转基因小鼠体内淋巴细胞表型特点及干扰素α对其病毒学指标和免疫细胞表型的影响。方法选取HBV转基因小鼠和野生型(WT)小鼠,采用ELISA检测HBV转基因小鼠血清HBs Ag和HBc Ab水平及两组小鼠血清IL-21及IL-6水平,分离肝、脾以及外周血淋巴细胞,流式细胞术检测CD4+T和CD19+B细胞频数;给予9只HBV转基因小鼠重组鼠源性干扰素α(rm IFN-α)皮下注射,同时9只HBV转基因小鼠相应注射PBS,观察其血清HBs Ag、HBV DNA、IL-6、IL-21水平的变化以及外周血CD4+T和CD19+B细胞频数变化。结果 HBV转基因小鼠的血清HBs Ag水平较高并能检测到HBc Ab,其血清IL-21及IL-6水平较WT小鼠显著升高(P0.05);HBV转基因小鼠外周血、肝脏以及脾脏淋巴细胞中CD4+T细胞频数较WT小鼠均明显低下(P0.05),但其肝脏CD19+B细胞频数明显高于WT小鼠(P0.05);HBV转基因小鼠肝内CD4+T细胞频数与血清HBs Ag水平呈负相关,而肝内CD19+B细胞频数与血清HBc Ab水平呈正相关;rm IFN-α处理可显著提高HBV转基因小鼠外周血CD4+T和CD19+B细胞频数以及血清IL-6水平(P0.05)。结论 HBV转基因小鼠体内淋巴细胞亚群频数异常,外源性干扰素α可通过调节淋巴细胞亚群频数发挥免疫调节作用。
[Abstract]:Objective to investigate the effect of hepatitis B virus (HBV) transgenic mice lymphocyte phenotype and interferon alpha on the virological and immunological cell phenotype. Methods HBV transgenic mice and wild-type (WT) mice were detected by ELISA HBV and HBc Ag transgenic mice serum HBs Ab level and two groups of mice serum IL-21 and IL-6 levels. The separation of liver, spleen and peripheral blood lymphocytes, flow cytometry and CD4+T frequency of CD19+B cells; 9 HBV transgenic mice given recombinant murine interferon alpha (RM alpha IFN-) subcutaneous injection, and 9 HBV transgenic mice injected with PBS, to observe the serum HBs Ag, HBV DNA, IL-6, change the level of IL-21 and CD4+T in peripheral blood and the frequency of CD19+B cells in HBV transgenic mice. Results the changes of serum HBs and Ag level can be detected HBc Ab, serum IL-21 and IL-6 levels were significantly elevated in WT mice (P0.05); HB V transgenic mice peripheral blood, liver and spleen lymphocytes of CD4+T cells in WT mice were significantly lower than the frequency (P0.05), but the frequency of CD19+B cells was significantly higher than that in the liver of WT mice (P0.05); HBV transgenic mice liver CD4+T cell frequency and serum HBs Ag level was negatively correlated to the positive frequency of CD19+B cells in the liver serum HBc and Ab level increased significantly; HBV transgenic mice peripheral blood CD4+T and the frequency of CD19+B cells and serum IL-6 level of RM IFN- (P0.05). The frequency of the alpha transgenic mice in vivo lymphocyte subsets HBV conclusion abnormal exogenous interferon alpha by regulating lymphocyte subsets frequency play a role in immune regulation.

【作者单位】：器官衰竭防治国家重点实验室//广东省病毒性肝炎研究重点实验室//南方医科大学南方医院感染内科;广州市解放军458医院全军肝病中心//转基因工程研究室;
【基金】：广东省自然科学基金(2015A030310440)
【分类号】：R512.62

【相似文献】