凉山州部分彝族人群2011年HIV耐药警戒线监测研究

发布时间：2018-02-09 18:31

  本文关键词： 彝族人群 艾滋病病毒 耐药性　出处：《中国艾滋病性病》2015年01期 　论文类型：期刊论文

【摘要】：目的分析凉山州部分彝族人群2011年艾滋病病毒1型(HIV-I)在未治疗的感染人群中耐药毒株变异情况,为指导抗病毒治疗、评价治疗效果、合理调整治疗方案和制定应对措施提供依据。方法对129例25岁以下新确诊的HIV阳性血清样本,用In-house方法进行基因型耐药检测。共91例扩增测序成功,检测耐药传播相关的耐药突变,并分析其相关的亚型及其比例。结果 91例样本中,没有针对PR区的主要耐药性基因突变,但有7例样本存在针对RT区耐药性基因突变,4例为交叉突变(T215Y和V179E),3例为单一突变(T215Y、V179D和P225H),导致对齐多夫定(AZT)和司坦夫定(D4T)不同程度的耐药。RT区耐药株流行率为5.43%(7/129),属于中等流行水平(5%~15%)。91例样本中,CRF07-BCⅡ型所比例最大,达91.21%(83/91),B亚型仅占8.79%(8/91)。结论凉山州部分彝族人群未经抗病毒治疗的新发现HIV-1感染者中,已出现pol区耐药突变株,且传播水平达中度流行状态,需进一步加强抗病毒治疗患者的用药督导,提高其用药依从性,并持续性监测该地区抗病毒治疗人群的耐药情况。
[Abstract]:Objective to analyze the variation of drug-resistant strains of HIV type 1 (HIV-1) in untreated infected population in 2011 in some Yi people in Liangshan prefecture, so as to guide the antiviral therapy and evaluate the therapeutic effect. Methods A total of 129 newly diagnosed HIV positive serum samples under 25 years of age were tested for genotype resistance by In-house method. 91 cases were successfully amplified and sequenced. Drug resistance mutations associated with drug resistance transmission were detected, and the related subtypes and their proportions were analyzed. Results there were no major drug resistance gene mutations targeted to PR region in 91 samples. However, there were 7 cases of resistance gene mutation in RT region. 4 cases were cross-mutated T215Y and 3 cases were single mutation T215YN V179D and P225HG, which resulted in resistance to azidovudine AZT and Staveldine D4T in different degree. The prevalence rate of resistant strains in RT region was 5.437r129G. The proportion of CRF07-BC 鈪，

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