脑泡状棘球蚴病绵羊实验模型建立与MR表现

发布时间：2018-02-16 04:31

本文关键词： 脑泡状棘球蚴病绵羊模型　出处：《新疆医科大学》2013年硕士论文　论文类型：学位论文

【摘要】：目的：通过接种鼠源性泡状棘球蚴感染羊脑，探讨建立羊脑泡状棘蚴（Cerebralalveolar echinococcosis，CAE）病实验模型的方法，为观察脑泡状棘球蚴病形态学变化、MRI表现及免疫学分析提供良好载体。方法：实验用健康新疆大尾绵羊10只，随机分成两组并编号，先用DWI序列（b=1000s/mm2）测量脑内各部位的ADC值，再用两样本的t检验分析确保实验动物脑组织结构的一致性。所有实验动物均在直视下颅骨定位穿刺，脑内接种泡状棘球蚴源头蚴混悬液约0.2ml，分别在第6、12、18个月后用MRI观察羊脑泡状棘球蚴生长状况，观察DWI及MRS表现，剖检后分析其形态学及病理学特点。结果：所有实验动物脑组织各部位的ADC值经统计软件分析后，P0.05，没有统计学意义，，脑部各测量部位之间没有明显的组织差异。本实验经MRI及病理证实，成功建模3只，成虫率30.00%（3/10），MRI检测3只均为单发病灶，T2WI表现为结节状不规则低信号，其中2例具有典型“煤炭样”改变，内间杂多发小囊泡样稍高信号；光镜下观察病灶区有大量淋巴细胞、嗜酸性粒细胞及浆细胞浸润，周围小血管闭塞并伴有炎性反应。2例MRI检查见线条样稍短T2信号，解剖后未见明显病灶，5例MRI未见异常表现。结论：人工接种鼠源性泡状棘球蚴原头蚴悬液建立绵羊脑泡状棘球蚴病模型具有可行性，方法简单，具有可重复性。
[Abstract]:Objective: to establish an experimental model of Cerebralalveolar echinococcosis (CAEE) disease in sheep by inoculation of rat-derived hydatid alveolar hydatid infection in sheep brain. Methods: ten healthy Xinjiang large tail sheep were randomly divided into two groups and numbered. The ADC values of various parts of the brain were measured with DWI sequence of 1000smm2. Then the t-test analysis of two samples was used to ensure the consistency of the brain structure of the experimental animals. About 0.2 ml of hydatid suspension was inoculated into the brain of Echinococcus alveolaris. MRI was used to observe the growth, DWI and MRS of Echinococcus alveolaris in sheep brain after 6 ~ 12 months and 18 months later, respectively. Results: the ADC values of brain tissues of all experimental animals were analyzed by statistical software (P0.05), and there was no statistical significance. There was no significant tissue difference between the measured sites of the brain. MRI and pathology confirmed that 3 rats were successfully modeled. The adult rate was 30.000 / 10 / 10. All of them were single lesions with irregular low signal intensity on T2WI. The typical "coal like" changes were found in 2 cases, with a little hyperintense signal of multiple vesicles in the interiors, and a large number of lymphocytes, eosinophils and plasma cells infiltrated in the lesion area under light microscope. Peripheral small vessel occlusion with inflammatory response was found in 2. 2 cases of MRI with line like short T 2 signal. Conclusion: it is feasible to establish the animal model of cerebral alveolar echinococcosis in sheep by artificial inoculation of mouse alveolar hydatid suspension, the method is simple and reproducible.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R445.2;R532.32

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