PCR-荧光探针法检测庆阳地区丙型肝炎病毒基因型

发布时间：2018-02-16 18:46

  本文关键词： PCR-荧光探针法 庆阳 丙肝 基因型　出处：《中国免疫学杂志》2017年10期 　论文类型：期刊论文

【摘要】：目的:应用PCR-荧光探针法分析庆阳地区丙型肝炎患者基因型,并对PCR-荧光探针法的各种性能进行评价。方法:收集庆阳地区289例各种丙型肝炎患者的临床资料和外周静脉血,采用PCR-荧光探针法检测其基因型,并和PCR-反向点杂交法、测序法进行比较。结果:289份HCV RNA阳性血清标本中,PCR-荧光探针法基因型及亚型检出率为99.3%(287/289),其中1b型139例(48.1%),2a型136例(47.1%),3a型7例(2.4%),3b型5例(1.7%),未分出型2例(0.7%)。PCR-荧光探针法的特异度和准确度为100%,重复性良好,并与PCR-反向点杂交法、巢式PCR测序法分型结果均一致,三种方法的一致率为98.2%(56/57),差异无统计学意义(P0.05)。1b基因型患者ALT、AST、PLT和HCVRNA(lg)水平均高于2a基因型患者,差异具有统计学意义(P0.05)。结论:庆阳地区HCV基因型呈现多基因型分布特点,主要为1b型和2a型,且2a型和1b型的比例相当,呈现出2a型比例升高,1b型下降的趋势;PCR-荧光探针法HCV基因分型敏感度和特异度高,方法简单,适合临床实验中应用。
[Abstract]:Objective: to analyze the genotypes of hepatitis C patients in Qingyang area by using PCR- fluorescence probe method and to evaluate the performance of PCR- fluorescence probe method. Methods: the clinical data and peripheral venous blood of 289 patients with hepatitis C in Qingyang area were collected. PCR- fluorescence probe method was used to detect its genotypes, and PCR- reverse dot hybridization was used to detect its genotypes. Results the detection rates of genotypes and subtypes of HCV RNA positive serum samples were 99.3% 287 / 289%, including 139 cases with 1b type 48.1% ~ 2a and 136 cases with 47.1% ~ 3a type, 7 cases with 2.43b type, 5 cases with 2.43b type, and 2 cases with 0.7% PCR-fluorescence probe method. Specificity and accuracy are 100, reproducibility is good, The results were consistent with those of PCR- reverse dot hybridization and nested PCR sequencing. The concordance rate of the three methods was 98.2 / 56 / 57. There was no significant difference in the levels of alt ASTN PLT and HCVRNANa-lg in patients with genotype P0.05. 1b, which were higher than those in patients with genotype 2a. Conclusion: the genotypes of HCV in Qingyang area are characterized by multiple genotypes, mainly 1b and 2a, and the proportion of 2a and 1b is the same. The results showed that the ratio of 2a to 1b increased and decreased. PCR- fluorescence probe method had high genotyping sensitivity and specificity, and the method was simple and suitable for clinical trial.
【作者单位】： 庆阳市人民医院检验科;
【基金】：甘肃省卫生行业科研计划项目(GSWSKY-2014-49)
【分类号】：R440;R512.63
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本文编号：1516193