2013～2014年广西风疹病毒分子流行病学分析

发布时间：2018-02-21 11:43

本文关键词： 风疹病毒分子流行病学基因型　出处：《中国疫苗和免疫》2015年06期 　论文类型：期刊论文

【摘要】：目的研究广西风疹病毒基因型别及特征,为预防和控制风疹提供分子流行病学基线数据。方法对2013~2014年广西出疹病例中风疹病毒核酸阳性的病原学标本,使用淋巴信号激活因子转染的非洲绿猴肾细胞(Vero Cell Transfected to Express the Human Signaling Lymphocyte Activation Molecule,Vero/SLAM)进行病毒分离,再应用逆转录-聚合酶链反应(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)对分离到的风疹病毒进行E1基因的945个核苷酸(8633~9577nt)片段扩增,并对其PCR产物进行序列测定和分析。结果从收到的148份风疹核酸阳性咽拭子中分离培养出119株风疹病毒株,其中20株为1E基因型,99株为2B基因型,来源于9个不同的地市。其中2B基因型风疹毒株与其他省2B基因型毒株的同源性达98.77%~99.86%。所有119株风疹分离株在N-型糖基化位点和抗原表位位点均未发生突变,6株分离株在血凝抑制位点和中和位点上发生了突变,且均为2B基因型。结论广西2013~2014年风疹病毒在人群中以1E基因型和2B基因型共存循环,2B基因型为优势基因型,1E基因型可能正在逐步被2B基因型所代替。E1基因氨基酸序列虽保守,但亦有些毒株发生突变,今后应继续加强与完善广西风疹病原学监测。
[Abstract]:Objective to study the gene types and characteristics of rubella virus in Guangxi, and to provide molecular epidemiological baseline data for the prevention and control of rubella. Methods the pathogenic specimens of rubella virus nucleic acid positive in Guangxi from 2003 to 2014 were collected. Vero Cell Transfected to Express the Human Signaling Lymphocyte Activation molecules were used to isolate the virus from African green monkey kidney cells transfected with lymphoid signal activator. Reverse transcription-polymerase chain reaction (reverse Transcription-Polymerase Chain reaction reverse reverse PCR) was used to amplify the 945 nucleotides of the E1 gene of the isolated rubella virus. Results 119 rubella virus strains were isolated from 148 rubella nucleic acid positive pharynx swabs, of which 20 were 1E genotypes and 99 were 2B genotypes. The homology of rubella virus strains of genotype 2B with that of other provinces was 98.777.99. 86. All 119 isolates of rubella did not mutate at N-type glycosylation sites and epitope sites. Mutation occurred at the inhibition and neutralization sites of hemagglutination. Conclusion the genotype of rubella virus in Guangxi from 2013 to 2014 may be replaced by genotype 2B instead of amino acid sequence of .E1 gene, although the genotype of 1e and coexisting circulating genotype of 2B is the dominant genotype. But there are some strains mutated, we should continue to strengthen and improve the monitoring of rubella etiology in Guangxi.
【作者单位】：广西壮族自治区疾病预防控制中心;
【基金】：广西壮族自治区卫生厅科学研究课题(合同号:Z2011274、Z2011273、S201310-05)
【分类号】：R511.2

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