慢性乙型重型肝炎患者外周血Th17和Treg细胞的表达

发布时间：2018-02-25 16:22

本文关键词： 慢性乙型重型肝炎 Th17细胞 Treg细胞　出处：《泰山医学院》2013年硕士论文　论文类型：学位论文

【摘要】：目的探讨慢性乙型重型肝炎和慢性乙型重度肝炎患者外周血中Th17细胞和Treg细胞及其相关因子表达水平间的关系。方法选择2010年1月～2012年6月我院收治的26例慢性乙型重型肝炎患者和20名慢性乙型重度肝炎患者作为研究对象；采集患者静脉血，采用流式细胞仪检测两组患者外周血Th17/Treg、Th17/CD4+T细胞和Treg/CD4+T细胞；应用RT-PCR检测外周血PBMC中RORγt和FOXP3mRNA表达水平；采用ELASE法检测患者外周血中PBMC细胞培养上清中IL-17和TGF-β含量。结果 1.慢性乙型重型肝炎组患者Th17/Treg、 Th17/CD4+T和Treg/CD4+T分别为5.18、4.29和5.6，慢性乙型重型肝炎组患者Th17/Treg、Th17/CD4+T和Treg/CD4+T分别为2.01、1.08和6.92，两组间比较差异具有统计学意义（P0.05）。 2.慢性乙型重型肝炎组患者RORγt和FOXP3浓度分别为0.528、0.516μg/μl，慢性乙型重度肝炎组分别为0.504、0.522μg/μl，两组样本浓度间比较差异无统计学意义（P0.05）；RORγt纯度分别为1.842和1.828，FOXP3纯度分别为1.881和1.834，均处于1.8～2.0范围内，各样本总RNA浓度和纯度满足实验要求；紫外透射仪下电泳条带上有三条清晰的带，，说明RNA完整性较好。患者外周血PBMC经培养后，RORγt和FOXP3mRNA表达量，慢性乙型重型肝炎组分别为1.08和0.70，慢性乙型重度肝炎组分别为0.65和1.18，两组之间有统计学差异（P0.05）。 3.外周血PBMC培养上清液中IL-17和TGF-β含量，慢性乙型重型肝炎组患者分别为41.60pg/ml和256.19pg/ml，慢性乙型重度肝炎组患者分别为32.48pg/ml和299.37pg/ml，两组之间有显著统计学差异（P0.01）。结论慢性乙型重型肝炎患者外周血Th17细胞被激活而Treg细胞被抑制；Th17/CD4+T显著升高、Treg/CD4+T显著降低，转录因子RORγt mRNA表达增加而FOXP3mRNA表达减少，IL-17水平升高而TGF-β水平降低，引起体内免疫抑制效应减弱、免疫炎性效应增强，导致肝脏炎症活动加剧，最终发展为重型肝炎。
[Abstract]:Purpose. To investigate the relationship between the expression levels of Th17 cells, Treg cells and related factors in peripheral blood of patients with chronic severe hepatitis B and severe hepatitis B. Method. From January 2010 to June 2012, 26 patients with chronic severe hepatitis B and 20 patients with severe chronic hepatitis B were selected as study subjects. The levels of ROR 纬 t and FOXP3mRNA in peripheral blood PBMC were detected by RT-PCR, and IL-17 and TGF- 尾 in the supernatant of PBMC cells in peripheral blood were detected by ELASE method. Results. 1. Th17 / Tregt Th17 / CD4 T and Treg/CD4 T were 5.184.29 and 5.6 in patients with severe hepatitis B, respectively, and Th17 / Tregt Th17 / CD4 T and Treg/CD4 T in patients with severe hepatitis B were 2.011.08 and 6.92, respectively. There was a significant difference between the two groups (P 0.05). 2. The concentrations of ROR 纬 t and FOXP3 in patients with severe hepatitis B were 0.528 渭 g / 渭 l and 0.504 渭 g / 渭 l, respectively. There was no significant difference in the purity of ROR 纬 t between the two groups. The purity of ROR 纬 t was 1.881 and 1.834, respectively, in the range of 1.82.0. The total RNA concentration and purity of each sample met the experimental requirements, and there were three clear bands on the electrophoresis band under UV transmission instrument, which indicated that the integrity of RNA was better. The expression of ROR 纬 t and FOXP3mRNA in peripheral blood PBMC of patients was increased after culture. The chronic severe hepatitis B group was 1.08 and 0.70, and the chronic severe hepatitis B group was 0.65 and 1.18 respectively. There was significant difference between the two groups (P 0.05). 3. The levels of IL-17 and TGF- 尾 in the supernatant of peripheral blood PBMC culture were 41.60 PG / ml and 256.19 PG / ml in patients with severe hepatitis B and 32.48 PG / ml and 299.37 PG / ml in patients with severe hepatitis B, respectively. There was a significant difference between the two groups (P0.01). Conclusion. In patients with severe hepatitis B, Th17 cells in peripheral blood were activated but Treg cells were inhibited. Th17 / CD4 T was significantly increased. The expression of transcription factor ROR 纬 t mRNA was increased, and the expression of FOXP3mRNA was decreased. The level of IL-17 was increased and the level of TGF- 尾 was decreased. The immunosuppressive effect in vivo is weakened, and the immune inflammatory effect is enhanced, leading to the intensification of inflammatory activity in the liver, which eventually develops into severe hepatitis.
【学位授予单位】：泰山医学院
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R512.62

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