海南省关节炎和神经系统疾病患者莱姆病调查

发布时间：2018-02-25 19:16

本文关键词： 莱姆病血清标本抗体检测病原学检测　出处：《中国人兽共患病学报》2015年04期 　论文类型：期刊论文

【摘要】：目的了解我国海南省关节炎或神经性疾病患者的莱姆病感染状况,并确认海南省是否存在莱姆病人。方法海南省三亚市人民医院提供血清542份,均为关节炎或神经性疾病患者。采用间接免疫荧光法(Indirect Fluorescent-Antibody Test,IFA)和免疫印迹法(Western Blot,WB)对血清标本进行莱姆病抗体检测;并采用巢式PCR法对WB抗体阳性的血清标本进行病原学检测。结果 IFA方法检测血清样本542份,54份阳性,阳性率为9.96%。应用WB法对上述54份IFA阳性血清样本进行检测,12份阳性。采用巢式PCR方法检测12份WB阳性样本,2份阳性,测序结果表明两份均为B.garinii基因型。结论本研究证实了海南省人群中存在莱姆病。建议当地医生在诊治关节炎和神经性疾病患者时,可以考虑其是否罹患莱姆病。
[Abstract]:Objective to investigate the infection status of Lyme disease in patients with arthritis or neuropathy in Hainan Province, and to confirm whether there are patients with Lyme disease in Hainan Province. Methods 542 serum samples were provided by the people's Hospital of Sanya City, Hainan Province. The serum samples were detected for Lyme disease antibody by indirect Fluorescent-Antibody test (IFA) and Western blotblotWB (Western blotblotWB). Nested PCR method was used to detect the pathogens of WB-positive serum samples. Results IFA method was used to detect 542 serum samples and 54 serum samples were positive. The positive rate was 9.96%. Using WB method, 12 IFA positive serum samples were detected and 12 WB positive samples were detected by nested PCR method. The sequencing results showed that both of them were B. garinii genotypes. Conclusion this study confirmed the presence of Lyme disease in Hainan population. It is suggested that local doctors should consider whether they are suffering from Lyme disease in the diagnosis and treatment of arthritis and neuropathy.
【作者单位】：海南省三亚市人民医院检验科;中国疾病预防控制中心传染病预防控制所传染病预防控制国家重点实验室;
【基金】：国家“十二五”传染病重大专项(2012ZX10004219和2013ZX10004-101) 海南省自然科学基金项目(814389) 三亚市医疗卫生科技创新项目(YW1244)~~
【分类号】：R514

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