人可溶性PD-1在肝病患者血清中表达检测及临床意义

发布时间：2018-03-01 20:46

本文关键词： 肝病 ELISA 可溶性PD-　出处：《免疫学杂志》2015年09期 　论文类型：期刊论文

【摘要】：目的利用自主研发的可溶性PD-1(soluble PD-1,s PD-1)检测试剂盒,检测肝病患者外周血中s PD-1表达水平并分析其临床意义。方法 40例初诊HBV感染患者,其中单纯HBV+患者13例、合并肝硬化患者17例以及合并肝癌患者10例。同时收集16例性别年龄匹配的健康人作为对照组。利用自主研发的PD-1 ELISA试剂盒分析s PD-1表达水平,并结合临床资料分析s PD-1与临床参数的相关性以及在鉴别诊断中的价值。结果 HBV感染患者体内s PD-1水平显著高于健康对照组(P0.05);单纯性HBV感染组、合并肝硬化组以及合并肝癌组s PD-1水平(μg·L-1)分别为4.755±1.554、0.940 2±0.231 9和0.766 5±0.349 1。各肝病患者组之间s PD-1水平存在显著性差异(P0.01);且单纯HBV感染组s PD-1水平显著高于合并肝硬化(P0.05)以及合并肝癌组(P0.01);肝硬化组和肝癌组两者之间s PD-1水平不存在显著差异(P0.05)。s PD-1水平与临床检验指标如T-BIL、D-BIL、I-BIL、AST以及ALT呈线性正相关,有统计学意义(P0.05);s PD-1与ALP、TP、H-ALB、UREA、Cr-s以及UA未见显著相关性(P0.05)。ROC曲线分析发现,s PD-1在对健康照组与单纯乙肝患者鉴别中具有显著差异(P=0.002)。结论 s PD-1在单纯HBV感染患者体内显著升高,并在疾病进程呈现动态变化。提示其在肝病进展中可能发挥着重要的调控作用。
[Abstract]:Objective to detect the expression of s PD-1 in peripheral blood of patients with liver disease and analyze its clinical significance by using soluble PD-1(soluble PD-1 PD-1) kit developed by ourselves. Methods 40 patients with newly diagnosed HBV infection, including 13 patients with HBV alone, were enrolled in this study. 17 patients with liver cirrhosis and 10 patients with liver cancer were collected as control group. The expression of s PD-1 was analyzed by PD-1 ELISA kit. The correlation between s PD-1 and clinical parameters and its value in differential diagnosis were analyzed. Results the level of s PD-1 in patients with HBV infection was significantly higher than that in healthy controls (P 0. 05). The levels of s PD-1 (渭 g 路L -1) in patients with liver cirrhosis and liver cancer were 4.755 卤1.5540.232 卤0.2312 and 0.766 5 卤0.349 1, respectively. There was significant difference in the levels of s PD-1 between patients with liver disease and patients with liver cancer, and the level of s PD-1 in patients with simple HBV infection was significantly higher than that in patients with liver cirrhosis (P0.05). There was no significant difference in the level of s PD-1 between liver cirrhosis group and liver cancer group. There was a linear positive correlation between the level of s PD-1 and clinical laboratory parameters such as T-BILA D-BILP I-BILAST and ALT. There was no significant correlation between P0.05 PD-1 and ALPX TPH-ALBUEAA Cr-s and UA. The analysis of P 0.05 PD-1 showed that there was a significant difference in the differentiation between healthy group and simple hepatitis B group. Conclusion the level of PD-1 in patients with HBV infection is significantly higher than that in patients with simple HBV infection. It is suggested that it may play an important regulatory role in the progression of liver disease.
【作者单位】：唐山市工人医院感染科;苏州大学附属第一医院临床免疫学实验室;苏州大学附属第一医院消化内科;
【基金】：国家自然科学基金(30930085)
【分类号】：R512.6

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