microRNA-122在HBV相关性肝癌中的表达及意义

发布时间：2018-03-05 05:12

本文选题：肝癌　切入点：microRNA-　出处：《广东医学》2017年06期 　论文类型：期刊论文

【摘要】：目的研究microRNA-122(mi R-122)在HBV相关性肝癌中的表达及其意义。方法分别提取47例肝癌组织(其中HBsAg阳性36例,HBsAg阴性11例)及癌旁正常组织的总miRNA,应用实时荧光定量RTPCR技术检测miR-122的表达,分析其表达情况及其与临床病理特征的关系。结果 HBsAg阳性肝癌组织中miR-122的表达比癌旁组织及HBsAg阴性肝癌组织明显下调,差异有统计学意义(P0.05),在肝癌组织中miR-122的表达量与患者性别、年龄、肿瘤大小无相关性(P0.05),但与肿瘤TNM分期及是否有脉管侵犯关系密切(P0.05)。结论 miR-122在HBV相关性肝癌组织中的表达明显降低,提示miR-122的低表达与HBV相关性肝癌发生、发展存在密切关系,且miR-122表达水平可能成为诊断及判断肝癌预后的新方法。
[Abstract]:Objective to study the expression and significance of microRNA-122(mi R-122 in HBV associated hepatocellular carcinoma (HCC). Methods Total miRNAs were extracted from 47 cases of HCC tissues (including 36 cases of HBsAg positive and 11 cases of negative HBsAg) and adjacent normal tissues. The expression of miR-122 was detected by real-time fluorescence quantitative RTPCR technique. Results the expression of miR-122 in HBsAg positive HCC was significantly lower than that in paracancerous tissues and HBsAg negative HCC tissues. The difference was statistically significant (P 0.05). The expression of miR-122 in HCC was correlated with the sex and age of the patients. There was no correlation between tumor size and tumor size (P 0.05), but it was closely related to tumor TNM stage and vascular invasion. Conclusion the expression of miR-122 in HBV related liver cancer is significantly decreased, suggesting that the low expression of miR-122 is closely related to the occurrence and development of HBV related hepatocellular carcinoma. The expression of miR-122 may be a new method for diagnosis and prognosis of HCC.
【作者单位】：中山市人民医院肿瘤中心化疗科;
【基金】：中山市科技计划项目(医疗卫生)(编号:2014A1FC067)
【分类号】：R735.7;R512.62

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