急性大剂量华支睾吸虫感染大鼠血清学指标和肝脏病理形态学改变的研究

发布时间：2018-03-09 00:33

本文选题：华支睾吸虫　切入点：血清学指标改变　出处：《中国病原生物学杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的观察急性大剂量华支睾吸虫感染大鼠模型中的肝脏病理形态学和血清相关指标的改变。方法 12只5~6周龄雌性Sprague-Dawley(SD)大鼠分为实验组和不感染对照组。实验组灌胃200个华支睾吸虫囊蚴/大鼠,采用改良加藤法检查粪便中虫卵。灌胃5周后剖杀,取肝脏,切片,HE染色后观察病理改变。剖杀前采血,分离血清,检测总胆红素(TBIL)、间接胆红素(DBIL)、碱性磷酸酶(ALP)、谷草转氨酶(AST)、谷丙转氨酶(ALT)、白细胞介素-6(IL-6)、肿瘤坏死因子(TNF-α)含量,用华支睾吸虫IgG抗体试剂盒检测感染特异性。结果实验组大鼠感染囊蚴30d左右粪便中均查到华支睾吸虫虫卵;肉眼见肝脏肿大、淤血,胆管堵塞明显,HE显示胆管扩张、管壁增厚明显,肝细胞肿胀,部分肝小叶结构破坏,胆管上皮细胞排列不规则水肿、坏死;血清TBIL、DBIL、ALP、AST、ALT、IL-6、TNF-α水平较对照组均显著增高(P0.05),并产生高水平的华支睾吸虫特异性IgG抗体。结论急性大剂量华支睾吸虫感染时肝脏病理形态学和血清指标显著性改变,表明存在严重的肝脏损伤,应予以重视。本研究对探讨人体急性感染华支睾吸虫的致病性具有一定借鉴。
[Abstract]:Objective to observe the changes of liver pathomorphology and serum related indexes in rats with acute high-dose clonorchiasis sinensis infection. Methods Twelve 5-week-old female Sprague-Dawley SD rats were divided into experimental group and non-infected control group. Stomach 200 metacercaria of Clonorchis sinensis / rat, The eggs in feces were examined by modified Kato method. After 5 weeks of gastric perfusion, the liver was taken, and the pathological changes were observed after HE staining. Blood samples were collected before dissection and serum was isolated. The contents of total bilirubin, indirect bilirubin, alkaline phosphatase, alanine aminotransferase (alt), alanine aminotransferase (alt), interleukin-6 (IL-6) and tumor necrosis factor TNF- 伪 (TNF- 伪) were measured. Results the eggs of Clonorchis sinensis were found in the feces of rats infected with cysticercaria for 30 days. The wall was thickened obviously, the hepatocytes were swollen, some hepatic lobules were destroyed, the epithelial cells of bile duct were arranged irregularly and edema and necrosis. The serum levels of TBIL-DBILA and ALPG-ALT6 TNF- 伪 were significantly higher than those of the control group (P 0.05), and a high level of specific IgG antibody against Clonorchis sinensis was produced. Conclusion there are significant changes in pathological morphology and serum parameters of the liver in patients with acute high-dose Clonorchis sinensis infection. This study is useful for the study of the pathogenicity of human acute infection with Clonorchis sinensis.
【作者单位】：桂林医学院寄生虫学教研室;桂林医学院实验动物中心;
【基金】：广西自然科学基金项目(No.2016GXNSFBA380099)
【分类号】：R532.23

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