阿片类毒品对HIV-1感染者PBMCs中TLR9基因表达的影响

发布时间：2018-03-10 16:42

  本文选题：阿片类毒品　切入点：HIV-　出处：《病毒学报》2015年02期 　论文类型：期刊论文

【摘要】：探讨滥用阿片类毒品(Opiates)对Ⅰ型艾滋病病毒(Human immunodeficiency virus,HIV-1)感染者外周血单个核细胞(PBMCs)中TLR9基因表达水平的影响,为阐明阿片类毒品促进HIV-1复制的作用机制奠定基础。首先在南宁、柳州、钦州市地区的美沙酮、艾滋病自愿咨询检测门诊招募对象,经研究对象知情同意,分为4组,即阿片类毒品滥用的HIV-1感染组(Opiates HIV(+)组)、阿片类毒品滥用非HIV-1感染组(Opiates HIV(-)组)、非阿片类毒品滥用的HIV感染组(Non-opiates HIV(+)组)和健康对照组(Control组),每组随机招募50人。其次,以问卷形式调查对象的人口学特征,并采集其外周静脉血,分离出PBMCs后提取RNA。最后采用qPCR、蛋白质印迹法(Western blot,WB)法检测4组人群PBMCs中TLR9的mRNA、蛋白表达水平。经调查发现4组人群在年龄、性别、民族、户籍所在地、婚姻状况、文化程度和吸毒年限等人口学特征方面差异均无统计学意义(P0.05)。Opiates HIV(+)与Non-Opiates HIV(+)组病毒载量中位数分别为4.450×103和3.977×103 cp/mL,差异有统计学意义(P0.05)。TLR9 mRNA相对表达量在Opiates HIV(+)、Non-Opiates HIV(+)、Opiates HIV(-)和Control组中分别为(2.13±1.59)×10-3、(3.66±2.22)×10-3、(1.96±1.42)×10-3和(7.66±4.87)×10-3。阿片类毒品滥用和HIV感染对TLR9的表达存在交互作用(F=25.91,P=0.000)。经单独效应分析,HIV阳性与阴性人群中,阿片类毒品滥用者TLR9相对表达量均明显低于非毒品滥用者(P0.05);在吸毒人群中,HIV阳性者与HIV阴性者两组间差异没有统计学意义(P0.05);在不吸毒人群中,HIV阳性者低于正常组,两组间差异有统计学意义(P0.05)。WB的结果显示,Opiates HIV(+)、Non-Opiates HIV(+)、Opiates HIV(-)三组TLR9蛋白的表达量均低于Control组。上述结果表明阿片类毒品能够下调HIV-1感染者PBMCs中TLR9的表达水平,提示阿片类毒品可能通过影响TLR9所介导的机体免疫效应,进而促进HIV-1的感染复制。
[Abstract]:To investigate the effect of opioid drug abuse (Opiateses) on the expression of TLR9 gene in peripheral blood mononuclear cells (PBMCs) of human immunodeficiency virus-1 (HIV-1) infected patients, and to lay a foundation for elucidating the mechanism of opioid drugs promoting HIV-1 replication. Methadone and HIV voluntary counseling and testing outpatient recruitment in Qinzhou area were divided into 4 groups with the informed consent of the subjects studied. The HIV-1 infection group of opioid drug abuse, the Opiates HIV () group, the opioid drug abuse non-#en1# infection group, the non-opioid drug abuse HIV infection group and the healthy control group were recruited at random with 50 persons in each group. The demographic characteristics of the subjects were investigated by questionnaire, and their peripheral venous blood was collected. PBMCs was isolated and extracted. At last, the mRNAs and protein expression levels of TLR9 in PBMCs were detected by qPCRand Western blotWB. the results showed that the four groups were in age, sex, nationality, domicile location and marital status. There was no significant difference in demographic characteristics such as educational level and drug abuse age. The median viral load of Non-Opiates and HIV group were 4.450 脳 10 ~ 3 and 3.977 脳 10 ~ 3 cp / mL, respectively. The difference was statistically significant in the relative expression of mRNA between Opiates non-Opiates and Opiates (Non-Opiates). In the Control group, 2.13 卤1.59) 脳 10-3 (3.66 卤2.22) 脳 10-3) 1.96 卤1.42) 脳 10-3 and 7.66 卤4.87) 脳 10-3 respectively. There was interaction between opioid drug abuse and HIV infection on the expression of TLR9. The relative expression of TLR9 in opioid drug abusers was significantly lower than that in non-drug abusers (P 0.05); there was no significant difference between the two groups in TLR9 positive and HIV negative in drug users; in non-drug abusers, the level of TLR9 positive was lower than that in the normal group. There was significant difference between the two groups in the expression of TLR9 protein in the three groups of Control. These results indicated that opioid drugs could down-regulate the expression of TLR9 in PBMCs of HIV-1 infected patients. The results suggest that opioid drugs may promote the replication of HIV-1 by affecting the immune effect mediated by TLR9.
【作者单位】： 广西医科大学公共卫生学院 广西艾滋病防治研究重点实验室;广西壮族自治区疾病预防控制中心消杀与媒介控制所;广西医科大学第一附属医院老年消化内科;
【基金】：国家自然科学基金资助项目(81171624) 教育部博导课题(20114503110003) 广西医科大学青年科学基金项目(GXMUYSF201210)
【分类号】：R512.91
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