浙江省首例本地感染牛带绦虫的诊断和治疗

发布时间：2018-03-11 09:39

本文选题：牛带绦虫　切入点：诊断　出处：《中国寄生虫学与寄生虫病杂志》2017年01期 　论文类型：期刊论文

【摘要】：目的对浙江省1例本地感染绦虫病例进行诊断和治疗,并确定其所感染绦虫的种类。方法收集患者发病、临床表现、诊治过程等信息,对患者开展流行学调查。采用肛周透明胶纸法检查虫卵。使用槟榔-南瓜子进行驱虫治疗,对患者排出的虫体进行形态学观察,采用PCR扩增虫体DNA的线粒体细胞色素C氧化酶1(cytochrome C oxidase 1,COX1)基因片段并进行序列分析。结果流行病学调查结果显示,患者近两年无县外出行史,无食生猪肉、牛肉或动物内脏史,但经常食用烧烤肉类及火锅,偶尔食用生蔬菜。患者肛周皮肤检查发现带绦虫虫卵。患者排出的虫体经形态学观察疑似为牛带绦虫(Taenia saginata)或亚洲带绦虫(Taenia asiatica)。PCR扩增COX1基因片段获得832 bp扩增产物,测序后经Bl AST比对分析显示,与牛带绦虫COX1基因(Gen Bank登录号为AB107239.1)序列相似性为99%,而与亚洲带绦虫(Gen Bank登录号为AB107235.1)和猪带绦虫(Taenia solium,Gen Bank登录号为AB066485.1)COX1基因序列相似性分别为96%和88%。结论综合患者的临床特征、流行病学信息和排出虫体的序列分析结果 ,确认该病例为本地感染牛带绦虫病例。
[Abstract]:The purpose of the diagnosis and treatment of 1 cases of local infection in Zhejiang province and to determine the type of tapeworm cases infected by Taenia. Methods patients, clinical manifestations, diagnosis process and other information, to carry out epidemiological investigation on patients with perianal. Using transparent adhesive tape method. Check the eggs anthelmintic treatment using areca - pumpkin seeds, morphology observation of the patient discharged from the body, by PCR amplification of Toxoplasma DNA mitochondrial cytochrome C oxidase 1 (cytochrome C 1 oxidase, COX1) gene fragments were sequenced and analyzed. The results of epidemiological investigation showed that patients with no history for nearly two years out, no fresh pork, beef or animal offal history, but often eat barbecue meat and Hot pot, occasionally eat raw vegetables. Patients with perianal skin findings of Taenia eggs. Larvae from patients suspected by observing the morphology of Taenia saginata (Taenia saginata) or Asia Tapeworm (Taenia asiatica).PCR COX1 gene fragment was amplified by 832 BP amplification, sequencing by Bl AST sequence analysis showed that, COX1 gene band and bovine tapeworm (Gen Bank accession No. AB107239.1) 99% sequence similarity, and Taenia asiatica (Gen Bank accession No. AB107235.1) and Taenia solium (Taenia solium, Gen Bank accession number AB066485.1) similar to the COX1 gene sequence was 96% 88%. conclusion and clinical characteristics of patients, epidemiological information and discharge sequences worm analysis results, confirming that the case for local infection of Taenia saginata cases.

【作者单位】：浙江省疾病预防控制中心;浦江县疾病预防控制中心;
【基金】：浙江省医药卫生科技项目(No.2014KYB324)~~
【分类号】：R532.3

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