评价荧光定量逆转录-聚合酶链反应和酶联免疫吸附试验方法在麻疹监测中的价值

发布时间：2018-03-12 06:20

本文选题：麻疹　切入点：酶联免疫吸附试验　出处：《中国疫苗和免疫》2015年06期 　论文类型：期刊论文

【摘要】：目的评价荧光定量逆转录-聚合酶链反应(real-time RT-PCR)和酶联免疫吸附试验(ELISA)方法在麻疹病毒感染病例中的诊断价值。方法分析2014年天津市麻疹监测信息报告管理系统报告数据,运用χ~2检验(Chi-square Test)、配对Mc Nemar检验和效度分析,比较2种方法的判定价值。结果 171例麻疹疑似病例同时采集了血清和干血片标本,ELISA法检测两种标本IgM抗体阳性率分别为53.22%和49.71%,差异无统计学意义(χ~2=3.60,P=0.06),两种标本ELISA法检测一致率为94.15%。2 512例麻疹确诊病例中,2 439例采集了血标本,ELISA法检测IgM抗体阳性率为76.38%;871例采集了咽拭子,real-time RT-PCR检测核酸阳性率为86.68%。咽拭子采样间隔在3天内的real-time RT-PCR核酸阳性率达到91.14%,3天后的阳性率只有66.26%(χ~2=72.46,P0.01)。血标本采样间隔在3天内的ELISA IgM抗体阳性率为71.47%,3天后达到94.14%(χ~2=118.06,P0.01)。检验效度分析,ELISA方法灵敏度为71.60%,特异度为85.29%,约登指数为0.57。real-time RT-PCR方法灵敏度为99.60%,特异度为67.65%,约登指数为0.67。结论 real-time RT-PCR方法更敏感,适合发病早期诊断,ELISA方法更适合发病中后期诊断。
[Abstract]:Objective to evaluate the diagnostic value of real-time RT-PCR and Elisa in measles virus infection cases. Using 蠂 ~ 2 test to test Chi-square test, paired MC Nemar test and validity analysis were used. Results the positive rates of serum and dry blood samples were 53.22% and 49.71, respectively. There was no significant difference between the two methods (蠂 ~ (23. 60) P ~ (0.06), ELISA). The positive rate of IgM antibody detected by Elisa in blood samples of 2 439 cases was 76. 38% and 871 cases were detected by real-time RT-PCR. The positive rate of real-time RT-PCR with throat swab sampling interval within 3 days was 86. 68%. The positive rate of nucleic acid was only 66.26 after 3 days. The positive rate of ELISA IgM antibody in blood samples with sampling interval within 3 days was 71.470.The positive rate of ELISA IgM antibody reached 94.14 after 3 days. The sensitivity of Elisa was 71.600.The specificity was 85.29.The Jordan index was 0.57.real-time RT-PCR prescription. The sensitivity of the method is 99.60 and the specificity is 67.65. The Yorden index is 0.67.Conclusion the real-time RT-PCR method is more sensitive. It is suitable for early diagnosis of disease and Elisa is more suitable for diagnosis of middle and late stage of disease.
【作者单位】：天津市疾病预防控制中心;
【基金】：天津市疾病预防控制中心科技基金项目(CDCKY1405)
【分类号】：R511.1;R446.6

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