福建省延平区及永安市致泻性大肠埃希菌监测分析

发布时间：2018-03-13 05:18

  本文选题：致泻性大肠埃希菌　切入点：多重PCR　出处：《中国人兽共患病学报》2017年04期 　论文类型：期刊论文

【摘要】：目的掌握福建省腹泻患者致泻性大肠埃希菌(diarrheagenic Escherichia coli,DEC)的流行特征,为防控腹泻病的流行和爆发提供依据。方法搜集2010-2015年延平区和永安市哨点医院腹泻病标本1 950份,接种于麦康凯(MaC)培养基,挑选平板上粉红色、光滑型菌落,采用常规分离培养和分子诊断技术相结合的方法分离鉴定致泻性大肠埃希菌,用SPSS 17.0对结果进行统计分析。结果致泻性大肠埃希菌共检出129株,总检出率为6.62%;EPEC检出65株,检出率为3.33%,其中aEPEC 61株,tEPEC4株;ETEC和EAEC均检出32株,检出率为1.64%;未检出EHEC和EIEC。男女DEC检出率差异无统计学意义(P0.05);将腹泻患者按照年龄分为5组,DEC检出率分别为7.03%、4.60%、3.70%、7.28%和6.21%,各组EPEC、ETEC和EAEC检出率差异无统计学意义(P0.05)。致泻性大肠埃希菌(DEC)检出率最高的月份为8月和9月;农村和城市DEC检出率差异无统计学意义(P0.05);两地区各类型DEC构成比差异无统计学意义(P0.05)。结论福建省DEC存在3种类型,以EPEC为主(其中aEPEC占绝对优势),8、9月是DEC高发月份,0~5岁婴幼儿组及成人组是DEC的高发人群,合理使用抗生素治疗DEC需要得到重视。
[Abstract]:Objective to study the epidemiological characteristics of diarrhea in diarrhea patients of Fujian Province, and to provide a basis for the prevention and control of diarrhoeal diseases. Methods 1,950 specimens of diarrhoeal diseases from 2010 to 2015 were collected in Yanping District and Qinling Hospital of Yongan City. Inoculated on Mac medium of Mac, selected pink and smooth colony on flat plate, isolated and identified cathartic Escherichia coli by conventional isolation, culture and molecular diagnostic techniques. Results A total of 129 strains of Escherichia coli were detected with SPSS 17.0. The total detection rate was 6.62 strains, and the detection rate was 3.33%. Among them, 61 strains of aEPEC were detected by aEPEC and 32 strains of EAEC were detected. There was no significant difference in the detection rate of EHEC and EIEC.There was no significant difference in the detection rate of DEC between men and women (P 0.05). According to age, the detectable rates of DEC were 7.03% and 6.21%, respectively. There was no significant difference in the detection rates of EAEC and EAEC between the two groups. The highest detection rate of Escherichia coli was in August and September. There was no significant difference in the detection rate of DEC between rural and urban areas (P 0.05), and there was no significant difference in DEC composition ratio between the two regions. Conclusion there are three types of DEC in Fujian Province. EPEC was the dominant factor (aEPEC was the dominant factor), September was the month of high incidence of DEC. The high incidence of DEC was in infants and adults. The rational use of antibiotics in the treatment of DEC should be paid more attention to.
【作者单位】： 福建省疾病预防控制中心;福建省人兽共患病研究重点实验室;
【基金】：福建省医学创新课题(No.2011-CXB-19)资助~~
【分类号】：R446.5;R516
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