CXCL9在结核患者中的表达及其在结核病诊断中的应用

发布时间：2018-03-14 18:03

本文选题：CXCL　切入点：结核病　出处：《广东医学》2015年23期 　论文类型：期刊论文

【摘要】：目的检测不同人群和不同标本CXCL9的表达情况,评价其在结核病诊断中的应用价值。方法采用多功能液相芯片分析系统(Luminex)技术检测19例结核患者和17例健康对照者血浆、51例结核性胸膜炎患者和36例非结核性胸膜炎患者的胸腔积液中CXLC9的表达水平。通过绘制ROC曲线,评价CXCL9对结核病的诊断效能。结果结核患者血浆中CXCL9的表达水平显著高于健康对照者(P0.001)。结核患者血浆中CXCL9表达水平在抗结核治疗后显著下降。结核患者胸腔积液CXCL9的表达水平显著高于非结核患者(P0.001)。结核患者胸腔积液中CXCL9的表达水平显著高于血浆中的表达水平(P0.001)。血浆CXCL9分辨结核患者与健康对照者的曲线下面积为0.860 7,截断点值的敏感度为70.59%,特异度为89.47%。胸腔积液CXCL9分辨结核性胸膜炎患者与非结核患者的曲线下面积为0.928 1,截断点值的敏感度为88.89%,特异度为90.20%。结论 CXCL9在结核患者中高表达,能有效地区分结核病,可作为用于诊断结核的生物标识。
[Abstract]:Objective to detect the expression of CXCL9 in different populations and specimens. Methods the multifunctional liquid chip analysis system was used to detect the plasma levels of 51 tuberculous pleurisy patients and 36 non-tuberculous pleurisy patients in 19 patients with tuberculosis and 17 healthy controls. The expression level of CXLC9 in pleural effusion of patients with meningitis. By drawing the ROC curve, Results the expression of CXCL9 in plasma of patients with tuberculosis was significantly higher than that of healthy controls (P 0.001). The expression of CXCL9 in plasma of patients with tuberculosis decreased significantly after anti-tuberculosis treatment. The expression level of CXCL9 in pleural effusion was significantly higher than that in plasma. The area under the curve of plasma CXCL9 to distinguish TB patients from healthy controls was 0.8607, and the expression level of CXCL9 in pleural effusion was significantly higher than that in plasma. The sensitivity of point value is 70.59 and the specificity is 89.47.The area under the curve of CXCL9 in distinguishing tuberculous pleurisy from non-tuberculous pleurisy is 0.928 1, the sensitivity of cut-off point is 88.89 and the specificity is 90.20. Conclusion CXCL9 is highly expressed in tuberculosis patients. It can be used as a biomarker for diagnosis of tuberculosis.
【作者单位】：广东省深圳市第三人民医院肺一科;
【基金】：广东省医学科研基金资助项目(编号:B2014354) 广东省深圳市基础研究计划基础研究项目(编号:JCYJ20130329171031738) 广东省深圳市结核病临床重点专科建设项目
【分类号】：R52

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