广东省不同亚型AIDS病人HIV-1 gp120 V3环基因变异及其利用辅助受体的情况

发布时间：2018-03-19 09:14

本文选题：艾滋病病毒Ⅰ型　切入点：辅助受体　出处：《中国艾滋病性病》2015年05期 　论文类型：期刊论文

【摘要】：目的了解广东省艾滋病病毒(HIV)主要流行亚型及V3环氨基酸的变异特点,并分析病毒株利用辅助受体的情况。方法收集2013-2014年期间,在广州市第八人民医院住院的艾滋病(AIDS)病人的血浆256份,巢式聚合酶链反应(Nested-PCR)方法扩增env基因C2-C3(HBX2:6972~7365)区并直接测序,用Geen tool软件将基因序列翻译为氨基酸序列进行比对和分析。结果共有214例(83.6%)扩增成功,其中143例(66.8%)为CRF01_AE;45例(21.0%)为B/C重组型;26例(12.1%)为CRF01_AE/B亚型。V3环顶端四肽基序,73.4%(157/214)为GPGQ,11.7%(25/214)为GPGR,8.9%(19/214)为GPGK,2.3%(5/214)为GQGQ,其他为(GPGG、GLGH、GPGS、GPGH)3.7%(8/214)。使用国际上普遍应用的4种软件(PSSMx4r5、SAAC+BLAS、PSSMsisi、Geno2pheno)分别对V3环顶端四肽基序利用辅助受体的情况进行评估。综合评估结果显示,132例(61.7%)利用CCR5,67例(31.3%)利用CXCR4。在15例(7.0%)无辅助受体评估结果的病例中,14例为CRF01_AE,1例为CRF01_AE/B。研究还发现,71.1%(32/45)B/C重组型的V3四肽基序单一使用PSSMsisi软件的评估结果,与4种软件综合评估结果的一致率为100%。结论目前广东省HIV-1的主要流行株仍是CRF01_AE,其V3环氨基酸高度变异,顶端四肽基序主要是GPGQ。对毒株利用辅助受体的情况,宜采取国际普遍使用的4种软件进行综合分析。
[Abstract]:Objective to investigate the main epidemic subtypes of HIV / HIV in Guangdong Province and the variation characteristics of V3 cyclic amino acids, and to analyze the utilization of coreceptor in the virus strains. A total of 256 plasma samples from patients with HIV / AIDS in Guangzhou 8th people's Hospital were collected and amplified by nested polymerase chain reaction (nested PCR) from HBX2: 6972 (7365) region of env gene, and sequenced directly. The gene sequence was translated into amino acid sequence by Geen tool software for alignment and analysis. Of these, 143 cases (66.8) are CRF01AEG 45 (21.0) (B / C recombination type 26 cases have 12.1g) is CRF01_AE/B subtype .V3 top quadripeptide motif 73.4% 157R 157214) GPGQ 11.7R 11.7R / 214) GPGGR8.9214) is GQGQ = 2.3m5214) GQGQQ, other GGGGG GHGGHGPGHGPGHGPGHGPGH3.7ng / 8214.The use of four internationally popular software PSSMx4rssiphus AC / ASPSSMsisiGeno2o1 / ASPSSMsisiGeno2o) is the apical tip of GQGQQQQQQQQQQQQ, other GQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQ (GQGQQQ). The other 26 are GQGQQQQQQQQQQQQQQQQQQQQQQQ. The results of the comprehensive evaluation showed that 132 cases (61.7%) were using CCR567 cases (31.3%) CXCR4. in 15 cases (7.0%) there were 14 cases of CRF01AEN (1 case of CRF01AEEN = CRF01AEP-B). The study also found that the recombinant V3 tetrapeptide group was 71.1% / 32% / 45%. The results of a single use of the PSSMsisi software, Conclusion at present, the main epidemic strain of HIV-1 in Guangdong Province is CRF01AEE, its V3 cyclic amino acid height variation, and the top tetrapeptide motif is mainly GPGQ.Using the auxiliary receptor to the virus strain, the results show that: 1. Four kinds of software which are widely used in the world should be adopted for comprehensive analysis.
【作者单位】：广州医科大学附属广州市第八人民医院传染病研究所;
【基金】：广东省科技计划项目(2012B031800336) 十二五国家科技重大专项(2012ZX10001003) 广州市科技计划项目(NO2011J4300092)~~
【分类号】：R512.91

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