青海地区藏族人群HBV易感基因IL-18的多态性研究

发布时间：2018-03-19 22:23

本文选题：白细胞介素18（IL-18）　切入点：基因多态性　出处：《青海大学》2013年硕士论文　论文类型：学位论文

【摘要】：目的：通过检测青海藏族人群乙肝患者血清中白细胞介素18（IL-18）的基因多态性，探讨藏族人群乙肝高发的原因和白细胞介素18（IL-18）在HBV易感性中的作用,并为乙肝病毒携带者早期的诊断、排查及对乙肝患者的治疗提供的理论依据。方法：选择本地藏族常驻人口，抽取250例乙肝患者全血5ml，根据乙肝五项的检测结果，选择与HBV感染者的年龄、性别匹配的134例健康者进行对照，针对IL-18的多态性位点，应用PCR-SSCP方法筛选其在藏族人群中的分布频率，最终通过基因测序给予鉴定；从筛选出来的IL-18基因进行统计学分析，计算藏族HBV感染者和健康人群中IL-18的分布频率，判断有无明显差异，比较分析SNP等位基因和各个血清型的相关关系。所获得的数据运用SPSS17.0软件进行统计分析：基因型频率及等位基因频率的比较、估算相对危险度。结果：1.-137G/C:病例组与对照组(1)三种基因型频率比较：X~2值1.079，P0.05。等位基因频率比较：X~2值0.123，P0.05。(2)logistic多因素分析：GG型OR值0.953,95%CI区间为0.517~1.757；CG型OR值0.980,95%CI区间为0.529~1.816；等位基因C型OR值1.110,95%CI区间为0.619~1.990。2.-607C/A:病例组与对照组(1)三种基因型频率比较：X~2值1.150，P0.05。等位基因频率比较：X~2值1.139，P0.05。(2)logistic多因素分析：:CC型OR值1.169,95%CI区间为0.747~1.830； CA型OR值1.046,95%CI区间为0.685~1.598；)AA型OR值0.772,95%CI区间为0.471~1.267；等位基因A型OR值1.176,95%CI区间为0.873~1.585。3.-105A/C:病例组与对照组(1)三种基因型频率比较：X~2值1.615，，P0.05。等位基因频率比较：X~2值1.615，P0.05。(2)logistic多因素分析：AA型OR值0.925,95%CI区间为0.510~1.679；AC型OR值0.981,95%CI区间为0.537~1.793；等位基因C型OR值1.166,95%CI区间为0.663~2.053。结论：IL-18的基因启动子-607C/A、-137G/C和基因编码区-105A/C基因多态性与乙肝易感性无明显相关性。
[Abstract]:Objective: to investigate the cause of high incidence of hepatitis B and the role of IL-18 in susceptibility to HBV by detecting the polymorphism of interleukin-18 (IL-18) gene in serum of Tibetan patients with hepatitis B in Qinghai, and to explore the causes of the high incidence of hepatitis B and the role of IL-18 in the susceptibility of hepatitis B in Tibetan population. It also provides theoretical basis for early diagnosis, screening and treatment of hepatitis B carriers. Methods: the whole blood of 250 patients with hepatitis B was collected from the resident population of local Tibetans. According to the test results of five items of hepatitis B, 134 healthy persons matched with age and sex of HBV infection were selected as controls, and the polymorphic loci of IL-18 were analyzed. PCR-SSCP method was used to screen the distribution frequency of IL-18 in Tibetan population, and the distribution frequency of IL-18 in Tibetan HBV infected and healthy population was calculated by statistical analysis of IL-18 gene. The correlation between SNP allele and each serotype was compared and analyzed. The data obtained were statistically analyzed by SPSS17.0 software: comparing genotype frequency and allele frequency to estimate relative risk. Results: 1: 1.-137G / C: case group compared with the control group (1) the ratio of the three genotypes: 1: X2 was 1.079 / P0.05. The allele frequency was 0.123GG = 0.123P 0.05.2Logistic multivariate analysis: the OR = 0.953 / 95CI = 0.517171.757 / C = 0.58095 / 95 CI = 0.529 / 1.816; the OR = 1.110 / 95CI = 0.6191.990.0.992.-607Cr = = 0.6191.992.-607Cr =:. Comparison of the three genotypes frequencies: 1. 150 P0.05. allelic frequency: 1. 139A: 1. 139A multivariate analysis of multivariate analysis of multivariate analysis: the OR value of: 1: CC was 1. 169 卤95 / CI = 0. 747 / 1.830; OR of CA type was 1.046 / 95 / CI = 0. 68555 / 1.598A = 0.77295 / 95 CI = 0. 471a = 1. 267a; OR value of allele type A = 0. 471a = 1. 267a. 1.176 / 95CI = 0.873-1.585.-105A / C: comparison of the three genotypes: the ratio of the three genotypes to that of the case group and the control group (1); the ratio of allele frequency to the ratio of 1. 615 to 1. 615; the multivariate analysis of multivariate analysis of multivariate analysis of multivariate analysis: the OR of 0. 925 95 CI was 0. 510 / 2. The OR of the AC type was 0. 981 / 95 / 95 CI was 0. 537 / 1.793.The OR value of allele C was 1. 16695 / 95 CI = 0. 663N / 2.053. Conclusion the gene promoter -607C / Agn-137G / C and the gene coding region -105A / C polymorphism of the gene of 1: IL-18 have no significant correlation with the susceptibility to hepatitis B.
【学位授予单位】：青海大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R512.62

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