血流感染洋葱伯克霍尔德菌多位点序列分型研究

发布时间：2018-03-20 19:56

本文选题：血流感染　切入点：洋葱伯克霍尔德菌　出处：《中华医院感染学杂志》2017年20期 　论文类型：期刊论文

【摘要】：目的探讨血流感染洋葱伯克霍尔德菌的同源性,以便更好地控制短时间内洋葱伯克霍尔德菌引起的院内感染。方法对医院2013年5-6月分离的15株血流感染洋葱伯克霍尔德菌的菌株应用多位点序列分型方法(MLST),聚合酶链式反应(PCR)扩增atpD、gltB、gyrB、recA,lepA、phaC和trp B7个管家基因片段,测序结果与MLST数据库中的数据比对分析,获得菌株各管家基因的编号和ST型,研究其同源性。结果 15株菌中,13株菌的7个管家基因完全相同,同时gyrB管家基因出现新的序列型,为新的序列型(ST)。15株洋葱伯克霍尔德菌被分为3个基因型,分别为ST482、ST608和一个新的ST序列,来自神经外科的13株菌为同一个ST型。结论医院短时间内洋葱伯克霍尔德菌引起的血流感染属医院感染暴发,神经外科的13株菌为同一ST型。其中13株菌的ST型不仅相同且是新的ST型,与另外两科室的菌株不同。MLST在洋葱伯克霍尔德菌的分子生物学分型中的应用,表现出了较高的分辨率,对医院洋葱伯克霍尔德菌血流感染暴发的调查有重要意义。
[Abstract]:Objective to investigate the homology of blood stream infection with Bacillus cepacia. In order to better control the nosocomial infection caused by Brockholderia cepacia in a short period of time. Methods the multisite sequence typing method was applied to 15 strains isolated from May to June in the hospital. PCR was used to amplify 7 housekeeping gene fragments of AlepAphaC and trp B3. The results of sequencing were compared with the data in MLST database, and the number and St type of housekeeping genes were obtained and their homology was studied. Results the 7 housekeeping genes of 13 strains of 15 strains were identical. At the same time, there was a new sequence type of gyrB housekeeping gene. The 15 strains of Burkholderia cepacia were divided into three genotypes, ST482SST608 and a new ST-sequence, respectively. 13 strains of bacteria from neurosurgery belong to the same St type. Conclusion the blood stream infection caused by Burkholderia cepacia in hospital is a nosocomial infection outbreak. The 13 strains of neurosurgery belong to the same St type, 13 of which are not only the same and new St type, but also different from those of the other two departments in the application of MLST in molecular biological typing of Birkholderia cepacia. It has a high resolution and is of great significance in the investigation of blood flow infection of Burkholderia cepacia in hospital.
【作者单位】：华北理工大学附属医院检验科;北京协和医院检验科;
【分类号】：R446.5;R51

【相似文献】