基于microRNAs调控的慢性HBV感染的分子机制

发布时间：2018-03-21 18:38

  本文选题：慢性HBV感染　切入点：微小RNA　出处：《世界华人消化杂志》2016年08期 　论文类型：期刊论文

【摘要】：目的:从microRNAs(miRNAs)调控角度揭示慢性乙型肝炎病毒(hepatitis B virus,HBV)感染的分子机制.方法:将符合标准的病例分为慢性HBV感染者组(含慢性乙型肝炎及慢性HBV携带者)与正常组,借助Agilent Human miRNA 8×60k微阵列芯片检测血浆中miRNAs表达谱,求得两组间的差异表达miRNAs谱(P0.05),借助miRNA生物信息学分析软件预测其靶基因并对靶基因进行GO功能富集分析和Pathway分析.结果:两组间的差异表达miRNAs共69条(P0.05),28条上调,41条下调;GO分析及Pathway分析得到其功能主要涉及生物黏附、转录正/负调控、生物合成过程的正/负调控、氮化合物的代谢过程的正/负调控、蛋白质定位、蛋白氨基酸的磷酸化、Notch信号传导途径、细胞凋亡、Wnt信号通路、Hedgehog信号通路、T细胞受体信号通路、MAPK信号通路、转化生长因子b信号通路、B细胞受体信号通路、ErbB信号通路、p53信号通路等.结论:慢性HBV感染受特异性mi RNAs调控,其调控涉及多个生命过程和信号通路.
[Abstract]:Objective: to elucidate the molecular mechanism of chronic hepatitis B virus infection from the perspective of microRN AsmiRNAs.Methods: the patients with chronic HBV infection (including chronic hepatitis B and chronic HBV carriers) and the normal group were divided into two groups. The expression of miRNAs in plasma was detected by Agilent Human miRNA 8 脳 60k microarray chip. The differentially expressed miRNAs profiles were obtained between the two groups. The target genes were predicted by miRNA bioinformatics analysis software, and the target genes were analyzed by go function enrichment analysis and Pathway analysis. Results: there were 69 differentially expressed miRNAs genes in the two groups. The down-regulation of go analysis and Pathway analysis showed that its function was mainly related to biological adhesion. Positive / negative regulation of transcription, positive / negative regulation of biosynthesis, positive / negative regulation of metabolic process of nitrogen compounds, protein localization, phosphorylation of protein amino acids, Notch signaling pathway, Apoptosis / Wnt signaling pathway / Hedgehog signaling pathway / T cell receptor signaling pathway / MAPK signaling pathway. Transforming growth factor b (TGFb) signaling pathway / B cell receptor pathway / p53 signaling pathway etc. Conclusion: chronic HBV infection is regulated by specific mi RNAs, which involves multiple life processes and signaling pathways.
【作者单位】： 成都中医药大学附属医院感染科;成都中医药大学;四川大学华西医院感染科;广元市中医院消化科;成都中医药大学附属医院医务部;成都中医药大学附属医院科研部;成都市传染病医院感染科;简阳市中医院消化科;
【基金】：国家自然科学基金资助项目,Nos.81202624,81102720~~
【分类号】：R512.62
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本文编号：1645072