结核与非结核分枝杆菌感染者血浆中差异表达microRNAs筛选

发布时间：2018-03-21 22:15

本文选题：microRNA　切入点：非结核分枝杆菌　出处：《现代预防医学》2016年07期 　论文类型：期刊论文

【摘要】：目的筛选结核分枝杆菌(tuberculosismycobacterium,MTB)与非结核分枝杆菌(non-tuberculosismycobacterium,NTM)感染肺部结核病患者血浆中差异表达microRNAs(miRNAs)分子。方法收集唐山市第四医院与石家庄第五医院2013.10-2014.10期间确诊的NTM感染肺结核患者19例作为NTM组,MTB感染肺结核患者37例作为MTB组;抽取EDTA抗凝血并分离血浆。从两组中分别取6例研究样本,采用miRNAs芯片筛选差异表达miRNAs,其余样本采用RT-q PCR技术进行后期验证。结果与MTB组相比,NTM组差异表达显著的miRNA共11个,其中上调的有8个,分别为miR-26a,miR-24,miR-222,miR-191,miR-155,miR-126,miR-122和let-7b;下调的有3个,分别为miR-767-3p、miR-1283、miR-1281。分别选择上调明显的3个上调和下调的miRNA进行RT-q PCR验证(has-let-7b、has-miR-155、has-miR-26a、has-miR-1281、has-miR-1283、has-miR-767),验证结果与芯片筛选结果具有较好的一致性。结论 MTB与NTM感染肺部结核病患者血浆中存在差异表达miRNAs分子。
[Abstract]:Objective to screen the differential expression of microRNAsmiRNAs-molecules in plasma of patients infected with pulmonary tuberculosis by Mycobacterium tuberculosismycobacterium tuberculosis (MTB) and non-tuberculous Mycobacterium tuberculosis (NTM). Methods the pulmonary nodule of NTM infection diagnosed between the fourth Hospital of Tangshan City and the Fifth Hospital of Shijiazhuang between March 10 and October 2014 was collected. Nuclear patients were treated as NTM group (n = 37), pulmonary tuberculosis group (n = 37) and MTB group (n = 37). EDTA anticoagulant was extracted and plasma was isolated. Six samples were collected from the two groups, and the differential expression of miRNASS was screened by miRNAs microarray, and the other samples were verified by RT-q PCR technique. Results compared with MTB group, there were 11 miRNA samples with significant differentially expressed miRNA. Eight of them, miR-26afmiR-24, miR-222, miR-191, miR-155, miR-126, miR-122 and let-7b, were down-regulated, and three were down-regulated. Three up-regulated and down-regulated miRNA were selected to verify the expression of miRNAs molecules in the plasma of patients with pulmonary tuberculosis infected with MTB and NTM respectively by RT-q PCR verification of has-let-7bsi-has-miR-155has-miR-1281Hhas-miR-1283has-miR-7671.Conclusion there is a difference in expression of miRNAs molecules between MTB and NTM infected patients with pulmonary tuberculosis.
【作者单位】：华北理工大学公共卫生学院;唐山市结核病医院检验科;石家庄第五医院检验科;
【分类号】：R52

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