外周血hsa-miR-342-5p诊断儿童结核的特异度和灵敏度

发布时间：2018-03-22 22:08

本文选题：微小RNA　切入点：生物标志　出处：《临床儿科杂志》2015年09期 　论文类型：期刊论文

【摘要】：目的评估外周血hsa-miR-342-5p检测对早期诊断儿童结核的价值。方法收集初治涂片阳性、涂片阴性结核患儿,肺炎患儿和正常儿童各20例,采集静脉血,TRIzol法提取外周血总RNA,q RT-PCR检测hsa-mi R-342-5p在各组间的相对表达水平,ROC曲线分析hsa-mi R-342-5p诊断儿童结核的特异度和灵敏度。结果 hsa-mi R-342-5p在涂片阳性结核、涂片阴性结核患儿、肺炎患儿和正常儿童四组间的差异具有统计学意义(F=21.94,P=0.00)。与正常儿童相比,hsami R-342-5p在涂片阳性结核、涂片阴性结核和肺炎患儿中的表达水平均下调,差异有统计学意义(P均0.01);与肺炎患儿相比,hsa-mi R-342-5p在涂片阳性结核、涂片阴性结核患儿中的表达也明显下调,差异有统计学意义(P0.01)。对于区分涂片阳性结核和肺炎,hsa-mi R-342-5p的特异度和灵敏度性均为90%;而区分涂片阴性结核和肺炎,其特异度和灵敏度分别为65%和100%。结论外周血hsa-mi R-342-5p有望成为新的分子指标用于儿童结核的早期诊断。
[Abstract]:Objective to evaluate the value of peripheral blood hsa-miR-342-5p in the early diagnosis of tuberculosis in children. Methods Twenty children with primary smear positive smear negative tuberculosis pneumonia and normal children were collected. The relative expression level of hsa-mi R-342-5p in peripheral blood was detected by collecting venous blood TRIzol method. The specificity and sensitivity of hsa-mi R-342-5p in diagnosis of children tuberculosis were analyzed by ROC curve. Results hsa-mi R-342-5p was positive for smear positive tuberculosis and negative for smear negative tuberculosis. The difference between the four groups of pneumonia and normal children was statistically significant. Compared with normal children, the expression levels of hsami R-342-5p in smear positive tuberculosis, smear-negative tuberculosis and pneumonia children were all down-regulated. The difference was statistically significant (P < 0.01), and the expression of hsa-mi R-342-5p in smear positive tuberculosis and smear-negative tuberculosis children was also down-regulated. The difference was statistically significant (P 0.01). The specificity and sensitivity of hsa-mi R-342-5p in differentiating smear positive tuberculosis from pneumonia were 90%, while those in smear negative tuberculosis and pneumonia were 90%. The specificity and sensitivity were 65% and 100% respectively. Conclusion hsa-mi R-342-5p in peripheral blood may be a new molecular marker for early diagnosis of tuberculosis in children.
【作者单位】：重庆医科大学附属儿童医院儿童发育疾病研究省部共建教育部重点实验室儿科学重庆市重点实验室重庆市(儿童发育重大疾病诊治与预防)国际科技合作基地;
【基金】：国家自然科学基金面上项目(No.81071406) 重庆市医学科研项目计划(No.20142046)
【分类号】：R529.9

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