脑脊液细胞中结核早期分泌性靶抗原6的检测及其早期诊断价值

发布时间：2018-03-26 17:51

本文选题：免疫荧光染色法　切入点：早期分泌性靶抗原　出处：《细胞与分子免疫学杂志》2015年03期

【摘要】：目的探讨免疫荧光染色法检测脑脊液细胞中结核早期分泌性靶抗原6(ESAT-6)对结核性脑膜炎诊断的价值。方法收集36例确诊结核性脑膜炎患者的脑脊液为病例组,45例非结核性脑膜炎患者脑脊液为对照组。用双盲法对2组脑脊液细胞进行ESAT-6免疫荧光染色,统计分析灵敏度和特异度等。结果病例组ESAT-6存在于中性粒细胞、单核细胞及淋巴细胞中,对照组脑脊液细胞中无ESAT-6表达。灵敏度为88.89%、特异度为93.33%;阳性预测值为91.43%、阴性预测值为91.30%;阳性似然比为13.33、阴性似然比为0.12;Youden指数为82.22%,ROC曲线下面积为0.94。结论免疫荧光染色检测脑脊液细胞中ESAT-6的灵敏度及特异度均较高,是结核性脑膜炎诊断的有效方法。
[Abstract]:Objective to investigate the value of immunofluorescence staining in detecting early secretory target antigen (ESAT-6) in cerebrospinal fluid (CSF) cells for the diagnosis of tuberculous meningitis. Methods the cerebrospinal fluid (CSF) of 36 patients with tuberculous meningitis was collected as the case group (n = 45) and non-tuberculous meningitis group (n = 45). Cerebrospinal fluid (CSF) of patients with tuberculous meningitis was used as control group. ESAT-6 immunofluorescence staining was performed on cerebrospinal fluid cells in two groups by double blind method. Results ESAT-6 was found in neutrophils, monocytes and lymphocytes. The positive predictive value was 91.43 and the negative predictive value was 91.30. The positive likelihood ratio was 13.33, and the negative likelihood ratio was 0.12. The area under the ROC curve was 0.94.Conclusion Immunofluorescence staining can be carried out in the cerebrospinal fluid of the control group with a sensitivity of 88.89 and a specificity of 93.33. Conclusion the positive predictive value is 91.43, the negative predictive value is 91.30, the positive likelihood ratio is 13.33, the negative likelihood ratio is 0.12 and the Youden index is 82.22. The sensitivity and specificity of ESAT-6 in cerebrospinal fluid (CSF) cells were higher. It is an effective method for diagnosis of tuberculous meningitis.
【作者单位】：第四军医大学西京医院神经内科;西安市第一医院神经内科;
【基金】：国家自然科学基金(81371334)
【分类号】：R529.3

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