泰安市感染性腹泻非伤寒沙门菌耐药监测及分型研究

发布时间：2018-03-30 17:32

本文选题：腹泻　切入点：沙门菌　出处：《中国人兽共患病学报》2017年04期

【摘要】：目的分离鉴定泰安市腹泻病沙门菌的血清型分布、耐药性及PFGE分子分型和同源性特点。方法对分离自泰安市腹泻病人的粪便标本中的31株沙门菌进行生化鉴定和血清分型,采用微量肉汤稀释法对其进行16种抗生素敏感试验,利用PFGE进行分子分型及聚类分析。结果 31株沙门菌分属于10种血清型,肠炎沙门菌占35.5%(11/31)为优势血清型;31株沙门菌对头孢西丁和亚胺培南100%敏感,对磺胺异唑的耐药率为77.42%(24/31);31株沙门菌按照100%的相似度可分为17个PFGE型。结论药敏显示所检测样本存在较为严重的多重耐药性,肠炎沙门菌遗传变异在该地区相对较为保守,且存在散发流行的可能,提示应做好肠炎沙门菌传染病的早期预警及防控。
[Abstract]:Objective to isolate and identify the serotype, drug resistance, PFGE molecular typing and homology of Salmonella spp.Methods 31 strains of Salmonella isolated from fecal specimens of diarrhea patients in Taian City were identified by biochemical method and serotyping. Sixteen antibiotic susceptibility tests were carried out by the method of broth dilution. Molecular typing and cluster analysis were performed by PFGE.Results 31 strains of salmonella belonged to 10 serotypes. Salmonella enteritidis was the dominant serotype. 31 strains were sensitive to cefoxitin and imipenem 100%.The resistance rate to sulfamisazole was 77.42% and 31 strains of Salmonella could be divided into 17 PFGE types according to the similarity of 100%.Conclusion there is serious multidrug resistance in the samples detected by drug sensitivity. The genetic variation of Salmonella enteritidis is relatively conservative in this area and there is a possibility of sporadic epidemic. It is suggested that the early warning and prevention and control of Salmonella enteritis infectious diseases should be done well.
【作者单位】：山东省泰安市疾病预防控制中心;莱芜市疾病预防控制中心;泰安市泰山区疾病预防控制中心;山东省疾病预防控制中心;
【基金】：国家科技重大专项课题(No.2012ZX10004-201-002)资助~~
【分类号】：R446.5;R516.3

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