云南省2012-2014年HIV-1流行毒株亚型分布特征

发布时间：2018-03-31 04:28

本文选题：艾滋病病毒-　切入点：流行毒株　出处：《中国艾滋病性病》2016年03期

【摘要】：目的研究云南省目前艾滋病病毒1型(HIV-1)流行毒株的亚型分布特点。方法收集2012-2014年云南省14个地市731例在定点医院随访的HIV感染者或艾滋病患者的血样及流行病学调查信息,采用反转录聚合酶链反应(RT-PCR)扩增HIV-1的3′半分子约4.5kb(包含env基因全长),序列校对后用Genotyping、MEGA6.06和BLAST工具软件确定毒株亚型,分析云南省HIV-1的地区和人群分布特征。结果共获得731例样本的224条半分子序列,各亚型HIV-1毒株及其所占的比例分别为CRF08_BC 58.0%,CRF01_AE 18.3%,CRF07_BC11.6%,未知重组8.0%,B(B′)亚型2.7%和C亚型1.3%。在德宏州和西双版纳主要以CRF01_AE为主,其余地州以CRF08_BC为主,在临沧、红河、玉溪、曲靖CRF08_BC占绝对优势(70%)。以上6种亚型的人口学特征(性别、年龄、婚姻状况、民族)及感染途径分布差异均无统计学意义(P0.05),B(B′)或C与CRF01_AE、B与C两种未知型重组在传播途径上的分布差异亦无统计学意义。结论运用3′半分子来确定病毒亚型,和前期2008-2009年的研究比较,云南省的HIV-1毒株在地域、人口学、传播途径等特征的分布上较之前有变化,仍有新型重组毒株出现,应密切监测流行趋势变化。
[Abstract]:Objective to study the subtype distribution of HIV-1 (HIV 1) in Yunnan Province. Methods Blood samples and epidemiological information of 731 patients with HIV infection or AIDS were collected in 14 cities of Yunnan Province from 2012 to 2014. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to amplify the 3 '-half molecule of HIV-1 (including the full length of env gene). The subtype of the virus was identified by genotype typing software MEGA6.06 and BLAST software. The regional and population distribution characteristics of HIV-1 in Yunnan Province were analyzed. The subtypes of HIV-1 virus strains and their proportions were CRF08_BC 58.0 and ARF01ACTF01ACTF01ACTF01AE18.33ACTF07A BC11.6, with an unknown recombination of 8.0% and 1.3%. In Dehong and Xishuangbanna, CRF01_AE was the main type, while the rest in Lincang, Honghe, Yuxi, Yuci, and Yuci, the rest of the states were CRF08_BC, ROC, Honghe, Yuxi, Xishuangbanna, Xishuangbanna, Xishuangbanna, and Lincang, Honghe, Yuxi, Qujing CRF08_BC is the dominant factor. Demographic characteristics of the above 6 subtypes (sex, age, marital status, etc.). There was no significant difference in the distribution of the two unknown types of recombination between C and CRF01AEB and C in the route of transmission. Conclusion the subtype of the virus is determined by using the 3 '-half molecule, and there is no significant difference in the distribution of the infection pathway between the two groups (P0.05) or CRF01AEB / C (P < 0.05) or CRF01AEB / C, respectively. Compared with the previous studies in 2008- 2009, the distribution of HIV-1 strains in Yunnan Province has changed in geographical, demographic and transmission characteristics, and there are still new recombinant strains, which should be closely monitored for the change of epidemic trend.
【作者单位】：云南省传染病专科医院艾滋病关爱中心;中国军事医学科学院微生物流行病研究所;
【基金】：云南省科技厅应用基础研究计划自筹项目(2013FZ217) 国家“十二五”传染病科技重大专项(2012ZX10001-002)~~
【分类号】：R512.91;R181.3

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