基于微卫星标记的印度中部Madhya Pradesh地区疟疾强化控制和非强化控制区的斯氏按蚊基因流和种群遗传结构分析（英

发布时间：2018-03-31 14:01

本文选题：斯氏按蚊　切入点：微卫星标记　出处：《昆虫学报》2015年01期

【摘要】：【目的】斯氏按蚊Anopheles stephensi是亚洲东南部城市人体疟疾的主要媒介,印度12%的疟疾病例由其引起。本实验研究了印度中部Madhya Pradesh地区东北部的疟疾强化控制(EMCP)区和非强化控制(非EMCP)区斯氏按蚊的基因流。在EMCP区,由于采用了各种疟疾防控措施因而疟疾病例首先降低,但是很快回升,说明总的疟疾风险维持稳定。【方法】应用7个微卫星位点,对印度中部Madhya Pradesh地区东北部的4个EMCP区和非EMCP区采集的斯氏按蚊进行基因分型,以分析各种群参数。【结果】发现各标记在所有种群中表现出高度的多态性。在两区间未发现很大的遗传多样性。观察到EMCP区的东部种群(FST=0.0485,RST=0.1112)比非EMCP区的北部种群(FST=0.020,RST=0.0145)具有较高的遗传分化,在EMCP区和非EMCP区之间观察到较高的基因流(12.90,6.16,5.06和2.38)。RST的灵敏度高于FST,说明分化可能是由于突变而非遗传漂变引起的。【结论】本研究表明,在EMCP区和非EMCP区内以及EMCP区和非EMCP区之间存在很高的基因流。基因流水平高以及抗虫性的发展似乎是EMCP区和非EMCP区疟疾病例发生增加的重要原因。
[Abstract]:[objective] Anopheles stephensi Anopheles stephensi is a major vector of human malaria in cities in southeastern Asia. In this study, we studied the gene flow of Anopheles stephensi in the northeast of Madhya Pradesh region in central India. As a result of various malaria control measures, malaria cases were first reduced, but recovered quickly, indicating that the overall malaria risk remained stable. [methods] Seven microsatellite sites were used. The genotypes of Anopheles stephensi collected from four EMCP and non-#en3# regions in the northeast of Madhya Pradesh region in central India were genotyped. In order to analyze various population parameters, it was found that the markers showed a high degree of polymorphism in all populations. No significant genetic diversity was found in the two regions. It was observed that the eastern population in the EMCP region was 0.0485 RST-0.1112) and the northern population in the non-#en1# region was 0.020 RST-0.0145). With high genetic differentiation, A higher sensitivity was observed between the EMCP region and the non EMCP region, indicating that the differentiation may be caused by mutation rather than genetic drift. [conclusion] this study shows that the sensitivity of FSTs is higher than that of FSTs. [conclusion] the present study suggests that the differentiation may be caused by mutation rather than genetic drift. [conclusion]. There is a high gene flow between the EMCP region and the non-#en1# region, and between the EMCP region and the non-#en3# region. The high level of gene flow and the development of insecticidal resistance seem to be the important reasons for the increase of malaria cases in EMCP and non-#en5# regions.
【作者单位】： Centre
【基金】：gratefully acknowledge the Department of Biotechnology,Govt. of India for providing financial assistance
【分类号】：R531.3;Q963

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