细菌性脑膜炎大鼠模型的尿液蛋白质组学变化

发布时间：2018-03-31 20:37

本文选题：细菌性脑膜炎　切入点：早期诊断　出处：《生物工程学报》2017年07期

【摘要】：与脑脊液和血液不同,尿液不受到稳态机制的调节,更倾向于积累和反应机体生理和病理状态下的变化。生物标志物的本质特征是变化,因此尿液是寻找疾病早期标志物的更好来源。在世界范围内,细菌性脑膜炎依然是引起新生儿和儿童疾病的主要原因。为了降低死亡率和致残率,需要用无创的方法寻找细菌性脑膜炎的相关线索。本研究中,使用大鼠脑室内注射大肠杆菌模型模拟细菌性脑膜炎,在第1天和第3天的大鼠尿液中寻找尿液蛋白谱的差异变化,为进一步寻找大肠杆菌性脑膜炎的早期生物标记物研究进行初步的探索。通过膜上酶切和胶内酶切将尿蛋白切成肽段并通过液相色谱串联质谱技术(LC-MS/MS)分析肽段信息。第1天的尿液通过胶内酶切方法鉴定到17个差异蛋白,通过膜上酶切鉴定到20个差异蛋白;第3天的尿液通过膜上酶切方法鉴定到5个差异蛋白。这些差异蛋白为寻找细菌性脑膜炎早期生物标志物的初步探索奠定了基础。
[Abstract]:Unlike cerebrospinal fluid and blood, urine is not regulated by the steady-state mechanism, and is more prone to accumulate and respond to physiological and pathological changes in the body.The essential feature of biomarkers is change, so urine is a better source of early disease markers.Bacterial meningitis continues to be the leading cause of neonatal and childhood disease worldwide.To reduce mortality and disability, noninvasive methods are needed to find clues to bacterial meningitis.In this study, the rat intraventricular injection of Escherichia coli model was used to simulate bacterial meningitis, and the difference of urine protein profile was found in the urine of rats on day 1 and day 3.To further explore the early biomarkers of Escherichia coli meningitis.Urine protein was digested into peptide segments by enzyme digestion and endonuclease digestion. The peptide segment information was analyzed by liquid chromatography tandem mass spectrometry (LC-MS / MS).On the first day, 17 differentially expressed proteins were identified by endonuclease digestion, 20 differential proteins were identified by endonuclease digestion on the membrane, and 5 differentially expressed proteins were identified by endonuclease digestion on the third day.These differential proteins lay a foundation for preliminary exploration of early biomarkers of bacterial meningitis.
【作者单位】：中国医学科学院基础医学研究所北京协和医学院基础学院病理生理学系;北京师范大学生物化学与分子生物学系北京基因工程与生物技术重点实验室;
【基金】：国家重点研发计划课题(No.2016YFC1306300) 国家重点基础研究发展计划(973计划)(No.2013CB530805) 北京自然科学基金(Nos.7173264,7172076) 中央高校基本科研业务费专项资金(No.2015KJJCB21) 北京合作建设项目(No.11065113) 北京师范大学(No.11100704)资助~~
【分类号】：R-332;R515.2

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