CYP1A1和GSTP1基因多态性与抗结核药物性肝损伤的关系

发布时间：2018-04-01 13:08

本文选题：细胞色素P　切入点：A　出处：《中国临床药理学杂志》2015年04期

【摘要】：目的分析细胞色素P450 1A1(CYP1A1)和谷胱甘肽转移酶P1(GSTP1)基因多态性与抗结核药物性肝损伤(ADLI)的相关性。方法抗结核治疗致肝损伤的结核病患者127例为病例组,127例无肝损伤者为对照组,收集其环境因素暴露资料及静脉血。CYP1A1基因Msp I位点和GSTP1基因Ile105 Val位点多态性检测用聚合酶链反应限制性片段长度多态法(PCR-RFLP)。用SPSS17.0软件对环境因素和基因型进行单因素和多因素条件Logistic回归分析。结果 CYP1A1基因Msp I位点各基因型在2组间的分布差异无统计学意义;GSTP1基因Ile105Val位点AA、AG、GG基因型在病例组和对照组频率分别为55.91%,32.28%,11.81%和70.08%,26.77%,3.15%,GG基因型在2组分布差异有统计学意义(P0.05)。GG基因型的危险度比值比(OR)值为4.701。单因素条件Logistic回归分析发现,饮酒为ADLI的危险因素;多因素分析中调整饮酒因素的影响后,GSTP1基因Ile105 Val位点GG基因型仍为ADLI的危险基因型。结论 GSTP1基因Ile105 Val位点多态性与ADLI的发生有关,携带GG基因型的个体是危险人群。
[Abstract]:Objective to investigate the association between cytochrome P450 1A1 (CYP1A1) and glutathione transferase (GSTP1) gene polymorphisms and anti-tuberculosis drug induced liver injury (ADLI).Methods 127 cases of tuberculosis patients with liver injury were treated with anti-tuberculosis. 127 cases without liver injury were treated as control group.The environmental exposure data and polymorphism of Msp I locus and Ile105 Val locus of CYP1A1 gene were detected by polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP).Univariate and multivariate conditional Logistic regression analysis was performed for environmental factors and genotypes with SPSS17.0 software.The risk ratio of P0.05. GG genotype was 4.701.Univariate conditional Logistic regression analysis showed that drinking was a risk factor for ADLI, and GG genotype at Ile105 Val locus of GSTP1 gene was still a risk genotype of ADLI in multivariate analysis.Conclusion the polymorphism of Ile105 Val locus of GSTP1 gene is associated with the occurrence of ADLI, and individuals with GG genotype are at risk.
【作者单位】：河北联合大学公共卫生学院河北省煤矿卫生与安全重点实验室;唐山市结核病医院检验科;
【基金】：国家自然科学基金资助项目(81041096) 唐山市重点实验室基金资助项目(08150201A-1-8)
【分类号】：R52

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