河南驻马店市性传播HIV感染者中流行的HIV亚型分析

发布时间：2018-04-02 13:32

本文选题：艾滋病病毒　切入点：性传播　出处：《中国艾滋病性病》2015年10期

【摘要】：目的分析河南驻马店市经性传播的艾滋病病毒(HIV)感染者中,HIV流行毒株亚型及其毒株特征。方法通过专业问卷调查,了解驻马店地区经性传播HIV感染者的基本状况。采集感染者的EDTA抗凝静脉血,检测其CD+4T淋巴细胞(简称CD4细胞)计数,提取外周血浆中病毒核糖核酸(RNA),使用反转录巢式荧光定量聚合酶链反应(PCR)方法扩增病毒全长gag和部分pol基因,利用Clustal软件比对序列,利用BioEdit软件手工校对,利用NCBI网站HIV分型工具分析病毒亚型,利用MEGA软件构建NJ树,分析病毒系统进化关系。结果共收集到HIV感染者血液74份,全部经性传播感染HIV。经过测序,共获得HIV全长gag基因56条(75.7%),全长pol基因38条(51.4%)。对61例HIV感染者感染病毒亚型进行了分析,其中B亚型毒株47例(77.0%),CRF01_AE毒株6例(9.8%),CRF07_BC毒株4例(6.6%),C亚型毒株2例(3.3%),新型重组毒株2例(3.3%)。系统进化分析显示,所有的B亚型毒株均为Thai B毒株。38例获得pol区序列的样本中,有4例具有耐药突变。结论驻马店市性传播HIV感染者中流行的HIV毒株构成复杂,Thai B亚型毒株是优势毒株,CRF01_AE、CRF07_BC、C等亚型毒株均已进入该人群,而且出现了新型重组毒株。针对该人群的HIV流行毒株监测至关重要。
[Abstract]:Objective to analyze the prevalence of HIV subtypes and their characteristics among sexually transmitted HIV infected persons in Zhumadian, Henan Province. Methods A professional questionnaire survey was conducted. To understand the basic status of sexually transmitted HIV infection in Zhumadian area. To collect the EDTA anticoagulant venous blood and to detect the CD4T lymphocyte (CD4 cell) count. Virus RNA RNA was extracted from peripheral plasma. The full-length gag and part of pol gene were amplified by reverse transcription nested fluorescence quantitative polymerase chain reaction (RT-PCR). The sequences were compared by Clustal software, and then manually proofread by BioEdit software. NCBI website HIV typing tool was used to analyze virus subtypes, MEGA software was used to construct NJ tree, and virus phylogenetic relationship was analyzed. Results 74 blood samples of HIV infected patients were collected, all of them were sexually transmitted HIV. A total of 56 HIV full-length gag genes and 38 full-length pol genes were obtained. The viral subtypes of 61 HIV infected patients were analyzed. Among them, 47 cases of subtype B virus (77.0%) and 6 cases of subtype B subtype AE (9.8%), 4 cases of CRF07BC strain, 2 cases of subtype C virus, 2 cases of new recombinant virus strain, 2 cases of pol region sequence, showed that all strains of subtype B were Thai B strains and 38 samples with pol region sequence. Conclusion the prevalence of HIV strains among sexually transmitted HIV infections in Zhumadian is the predominant strain of the complex Thai B subtype, which is the dominant strain. All the subtypes of CRF01AECRF07BCCnC have entered this population. [WT5HZ] [WT5 "HZ] [WT5" BZ] [WT5 "HZ] [WT5" BZ] [WT5 "BZ]. Moreover, new recombinant strains have emerged. Surveillance of HIV epidemic strains in this population is very important.
【作者单位】：河南省疾病预防控制中心;军事医学科学院微生物流行病研究所全军艾滋病检测中心病原微生物生物安全国家重点实验室;驻马店市疾病预防控制中心;
【基金】：国家科技重大专项(2012ZX10001-002) 国家自然基金项目(81273137)~~
【分类号】：R512.91

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