一株达菲耐药的甲型H1N1流感病毒全基因特征分析

发布时间：2018-04-09 22:09

本文选题：甲型HN流感病毒　切入点：达菲耐药　出处：《中国微生态学杂志》2016年08期

【摘要】：目的分析1株甲型H1N1流感达菲耐药病毒株的全基因特征,为指导流感临床治疗与防控提供依据。方法采用鸡胚进行病毒分离,提取病毒RNA,通过RT-PCR扩增其全基因组8个基因片段,测定核苷酸序列,利用生物信息软件拼接全基因组序列,绘制基因进化树并分析重要基因位点变异情况。结果 A/Fuzhou/SWL11609/2013(H1N1)流感毒株的8个节段基因均处于2013-2014年度进化簇中,且与A/California/07/2009(H1N1)疫苗株高度同源,8个基因同源性均在97.4%以上;其HA和NA基因与A/Hubei-Wuchang/SWL1322/2013(H1N1)达菲耐药株同源性最高,分别为100.0%和99.6%;初步判断该毒株未发生重配现象,其重要致病性基因位点未发生变异;NA基因中具有H275Y典型达菲耐药位点特征,缺失一个糖基化位点(N386K),降低了基因结构的稳定性,同时在V241I和N369K的作用下降低了病毒的适应性。结论本次研究的甲型H1N1流感达菲耐药病毒株表现为低致病性流感病毒特征,但具备较好的人传人能力,需进一步加强监测,谨防耐药株的广泛流行。
[Abstract]:Objective to analyze the genetic characteristics of 1 strains of influenza a H1N1 influenza Tamiflu resistant strains, provide the basis for clinical treatment and prevention of influenza. Methods the chicken virus was isolated, viral RNA extracted from the whole genome of 8 gene fragments amplified by RT-PCR, sequenced, using whole genome bioinformatics software splicing, phylogenetic the tree and analysis of important gene variation. The results of A/Fuzhou/SWL11609/2013 (H1N1) flu strain of 8 segments which are in the 2013-2014 year evolution of gene clusters, and A/California/07/2009 (H1N1) vaccine strains are highly homologous, 8 genes homology was more than 97.4%; the HA and NA gene and A/Hubei-Wuchang/SWL1322/2013 (H1N1) of Tamiflu resistant strains the highest homology were 100% and 99.6%; the preliminary judgment of reassortment happened on the virus, its important pathogenic gene loci did not mutate; NA H275Y has the typical characteristics of Tamiflu resistance loci gene, deletion of a glycosylation site (N386K), reduced the stability of gene structure, and lower the virus adaptability in V241I and N369K. The conclusion of the study of the H1N1 influenza virus strains showed Tamiflu resistant low pathogenic characteristics of influenza virus. But good person ability, need to further strengthen the monitoring, beware of popular drug resistant strains.

【作者单位】：福建省疾病预防控制中心福建省人兽共患病研究重点实验室;福建医科大学公共卫生学院;
【基金】：福建省自然科学基金(2015J01294) 福建省医学创新课题(2014-CX-9)资助福建省卫生系统中青年骨干人才培养项目(2015-ZQN-ZD-10)
【分类号】：R511.7

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