一起旅游团中急性胃肠炎的暴发与GII. 17型诺如病毒相关

发布时间：2018-04-11 18:49

本文选题：诺如病毒(Norovirus) + 急性胃肠炎　；参考：《病毒学报》2017年05期

【摘要】：为了解2016年夏季一起发生在某赴内蒙古旅游团游客中的急性胃肠炎暴发流行的病毒病原。分别采集此次胃肠炎暴发流行中两名患病游客腹泻症状发生后第3天、5天和7天的粪便标本,提取核酸后首先用荧光定量PCR扩增诺如病毒核酸进行检测,阳性标本分别用RT-PCR扩增病毒VP1基因和RdRp区域基因,通过基因测序和序列比对进行基因分型,构建进化树确定进化同源性。通过荧光定量PCR法检测到两名患者的粪便标本均为诺如病毒阳性,提示此次胃肠炎暴发流行的病原为诺如病毒,进一步的RT-PCR扩增病毒基因分析显示检测到的诺如病毒为GII.P17-GII.G17型;构建系统进化树分析显示本次与2014~2015年度广州、中国香港、中国台湾和日本等地的病毒亲缘关系更为接近;氨基酸序列分析显示该基因型与2014年以来流行的毒株同源性较近,与2014年之前流行的毒株相比变异较大,氨基酸变异主要出现在主要衣壳蛋白的P结构域。提示GII.P17-GII.G17型诺如病毒也是引起内蒙地区急性胃肠炎暴发流行的病毒病原。
[Abstract]:In order to understand the viral pathogens of acute gastroenteritis outbreak in a tourist group in Inner Mongolia in summer 2016.Stool samples of two tourists with diarrhea symptoms during the outbreak of gastroenteritis were collected respectively on the 3rd and 7th days after the onset of gastroenteritis. The nucleic acids were extracted and detected by fluorescence quantitative PCR amplification of norovirus nucleic acid.RT-PCR was used to amplify the VP1 gene and the RdRp region gene of the virus respectively. The gene was sequenced and sequenced to determine the evolutionary homology.The stool samples of the two patients were positive for norovirus by fluorescence quantitative PCR, indicating that the pathogen of the outbreak of gastroenteritis was Norovirus. Further RT-PCR amplification of the virus gene analysis showed that the detected norovirus was GII.P17-GII.G17 type.The phylogenetic tree analysis showed that the phylogenetic relationship was closer to that in Guangzhou, Hong Kong, China, Taiwan and Japan, and the amino acid sequence analysis showed that the genotype was close to that of the virus strains that had been prevalent since 2014.Compared with the prevalent strains before 2014, the variation of amino acids mainly occurred in the P domain of the main capsid protein.It is suggested that GII.P17-GII.G17 type Norovirus is also the pathogen causing the outbreak of acute gastroenteritis in Inner Mongolia.
【作者单位】：首都儿科研究所病毒研究室儿童病毒病病原学北京市重点实验室;
【基金】：北京市科委项目(项目号:Z11110705056811041),题目:北京儿童急性传染病监测体系的建立~~
【分类号】：R512.5

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