2012—2014年上海市蜱和宿主动物新型布尼亚病毒感染状初步调查

发布时间：2018-04-12 11:39

本文选题：发热伴血小板减少综合征 + 蜱　；参考：《中国人兽共患病学报》2017年08期

【摘要】：目的掌握上海市蜱分布及携带新型布尼亚病毒情况,了解上海市蜱动物宿主以及动物感染病毒状况,从而评估上海地区是否存在新型布尼亚病毒的传播风险。方法采用布旗法捕捉室外"游离蜱",动物"寄生蜱"采用动物体表采集法捕获犬体表蜱类样本;蜱病毒核酸的检测采用荧光定量PCR法;动物血清抗新型布尼亚病毒的总抗体检测采用双抗原夹心法。结果 2012—2014年期间,上海松江辰山和金山大金山岛监测到室外"游离蜱",蜱种均为长角血蜱;上海17个区中10个区监测到"寄生蜱",主要分布在崇明、奉贤、闵行等区,血红扇头蜱为优势蜱种。检测的143只蜱中,未发现病毒核酸。上海"寄生蜱"的动物宿主主要为犬,其次为羊,分布在6个主城区的198只犬、浦东新区和奉贤区的120份猪和崇明区的36头羊中,均未发现感染病毒。结论 2012—2014年上海地区蜱尚未发现新型布尼亚病毒的携带,动物监测中未发现感染。因此,尚无证据证实上海地区为新型布尼亚病毒自然疫源地,需进一步调查研究证实。
[Abstract]:Objective to investigate the distribution and transmission of new Bunia virus in ticks in Shanghai, and to understand the host and infection status of ticks in Shanghai, so as to evaluate whether there is a risk of transmission of new Bunia virus in Shanghai.Methods the outdoor "free ticks" were captured by the flag cloth method, the canine surface ticks samples were captured by the animal "parasitic ticks" method, and the detection of ticks viral nucleic acid was detected by fluorescence quantitative PCR method.The total antibody of anti-new Bunia virus in animal serum was detected by double-antigen clamp method.Results during 2012-2014, outdoor "free ticks" were detected in Shanghai's Songjiang Chenshan and Jinshanduanshan Island, with tick species of Haemaphysalis longicornis, and parasitic ticks were detected in 10 of 17 districts in Shanghai, mainly distributed in Chongming, Fengxian, Minhang, etc.Hematophorus hematidis is the dominant tick species.No viral nucleic acid was found in 143 ticks.The animal host of "parasitic ticks" in Shanghai was mainly dogs, followed by sheep, 198 dogs distributed in 6 main urban areas, 120 pigs in Pudong New area and Fengxian District and 36 sheep in Chongming District. None of them was infected with the virus.Conclusion A new Bunia virus was not found in Shanghai from 2012 to 2014, and no infection was found in animal surveillance.Therefore, there is no evidence to confirm that Shanghai is the natural focus of Bunia virus.
【作者单位】：上海市疾病预防控制中心;上海市松江区疾病预防控制中心;
【基金】：上海市第四轮公共卫生三年行动计划传染病与病原微生物学重点学科资助项目(No.15GWZK0101) 上海市第四轮公共卫生三年行动计划高端海外研修团队培养计划资助项目(No.GWTD2015S02) 长三角区域重大突发性传染病跨境公共卫生安全保障技术的开发及示范应用(No.16495810201)联合资助~~
【分类号】：R511

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