结核分枝杆菌TB-SA抗体检测试剂盒（胶体金法）对于结核病的诊断价值评估

发布时间：2018-04-12 12:40

本文选题：结核分枝杆菌表面抗原蛋白 + 结核抗体　；参考：《重庆医科大学》2013年硕士论文

【摘要】：研究目的：研究和评估赛普克（CYPCO）-结核分枝杆菌TB-SA抗体检测试剂盒（胶体金法）诊断结核病的特异性及敏感性，为临床推广应用提供依据。研究方法：拟选结核病患者、非结核的其他肺部疾病疾病患者及健康志愿者共计250例，分为五组分别为菌阳肺结核组、菌阴肺结核组、肺外结核组、其他疾病组及健康对照组。各组均进行血清及全血标本的结核分枝杆菌TB-SA抗体检测及同类参比试剂的平行检测。研究结果：血清结核分枝杆菌TB-SA抗体检测诊断结核病的总体灵敏度为76%，特异性为64%；全血结核分枝杆菌TB-SA抗体检测诊断结核病的总体阳性率为36%，，特异性为88%。研究结论：赛普克结核分枝杆菌TB-SA抗体检测试剂盒对于血清标本的检测灵敏度较全血标本高。综合其灵敏度与特异性，并与同类参比试剂相比，其具有良好的临床应用价值，值得临床应用与推广。
[Abstract]:Objective: to study and evaluate the specificity and sensitivity of CyPCO-Mycobacterium tuberculosis TB-SA antibody detection kit (colloidal gold method) in the diagnosis of tuberculosis, and to provide the basis for clinical application.Methods: a total of 250 tuberculosis patients, non-tuberculosis patients with other pulmonary diseases and healthy volunteers were divided into five groups: bacillus positive pulmonary tuberculosis group, bacterial negative pulmonary tuberculosis group, extrapulmonary tuberculosis group, other disease group and healthy control group.TB-SA antibody of Mycobacterium tuberculosis in serum and whole blood samples and parallel detection of similar reference reagent were carried out in all groups.Results: the total sensitivity and specificity of TB-SA antibody detection in diagnosis of tuberculosis were 760.The total positive rate and specificity of detection of TB-SA antibody against Mycobacterium tuberculosis in whole blood were 36g and 88g respectively.Conclusion: the sensitivity of TB-SA antibody detection kit for Mycobacterium tuberculosis is higher than that in whole blood samples.Compared with the similar reference reagent, it has good clinical application value and is worthy of clinical application and popularization.
【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R446.6;R52

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