寨卡病毒检测试剂盒对赣州市输入性寨卡病毒感染病例标本核酸的检测验证

发布时间：2018-04-13 15:39

本文选题：寨卡病毒 + 荧光PCR　；参考：《现代预防医学》2017年07期

【摘要】：目的验证商品化寨卡病毒核酸测定试剂盒,建立赣州市寨卡病毒的检测方法,为寨卡病毒疫情的判定及制定防控措施提供依据。方法用商品化寨卡病毒核酸测定试剂盒,对中国CDC检测定性的6份标本进行回顾性检测,用荧光定量PCR方法检测病毒核酸拷贝数浓度。对寨卡阳性标准品(1×10~7copies/ml)和以10倍梯度稀释为10~(-1)至10~(-7)倍共8个浓度梯度分别检测,验证试剂盒敏感性,对Ct值和拷贝浓度进行拟合获得标准曲线。对麻疹、风疹、登革热、肠道EV71型和肠道CA16型病毒进行核酸定性检测,验证试剂盒特异性。结果敏感性验证实验最低检测限约为1×10~3copies/ml;对麻疹等5种病毒检测定性为阴性;6份标本中病毒拷贝数为:1.9×10~4copies/ml、0、2.7×10~5copies/ml、0、0、0;且阴阳性对照和内部对照均成立。结论商品化试剂盒对本次寨卡病毒阳性血清和尿液标本可以定性检出,符合预期,对其他5种病毒无交叉反应。有一定的敏感性和特异性。试剂盒可以作为本市寨卡病毒的初步筛查检测,可用于寨卡病毒疫情的初步判定及防控。
[Abstract]:Objective to verify the commercialization of Zika virus nucleic acid detection kit and detection method of the establishment of Ganzhou city Zika virus, determine and provide a basis for formulating prevention and control measures of Zika virus outbreak. Methods the commercialization of Zika virus nucleic acid test kit for detection of CDC, China qualitative 6 samples were detected, copy the number of viral nucleic acid detection concentration by fluorescence quantitative PCR method. The village card positive standard (1 x 10~7copies/ml) and 10 fold dilution of 10~ (-1) to 10~ (-7) a total of 8 times the concentration gradient detection kit respectively, verify the sensitivity of the Ct value and concentration were obtained through fitting the standard curve. For measles, rubella, dengue fever, nucleic acids were qualitatively detected intestinal EV71 and intestinal CA16 virus test kit, specificity. Results the sensitivity experiments of the minimum detection limit is about 1 * 10~3copies/ml; 5 of measles virus detection as Yin Of the 6 specimens; the virus copy number: 1.9 * 10~4copies/ml, 0,2.7 * 10~5copies/ml, 0,0,0; and the Yin and Yang of control and internal control were established. Conclusion the commercial kit of this Zika virus positive serum and urine samples can be detected by qualitative analysis, in line with expectations, and no cross reaction with other 5 kind of virus. The sensitivity and specificity of the preliminary screening test kit can be used as the city Zika virus, can be used for preliminary judgement and prevention of Zika virus epidemic.

【作者单位】：赣州市疾病预防控制中心;
【分类号】：R440;R511

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