用megaTAL核酸酶对原代人T细胞CCR5基因座进行有效修饰可建立HIV-1抵抗力

发布时间：2018-04-14 14:22

  本文选题：T细胞CCR基因 + megaTAL　； 参考：《中国病理生理杂志》2017年02期

【摘要】：正已有研究表明,HIV-1协同受体(co-receptor)CCR5基因中自然发生的32个碱基对缺失(32-base pair deletion)可保护人CD4~+T细胞,对抗HIV感染。最近用工程化核酸酶干扰该基因和模拟此突变的基因工程方法展示了HIV治疗获得成功的迹象。Romano Ibarra等研制了一个靶向CCR5基因第3细胞外茎环(extracellular loop)的megaTAL核酸酶,并通过mRNA转染
[Abstract]:It has been shown that 32 base pair deletion (32-base pair deletion) in the co-receptor CCR5 gene of HIV-1 can protect human CD4T cells against HIV infection.Recently, a megaTAL nuclease targeting the extracellular loop of the third cell of CCR5 gene was developed by using engineering nuclease to interfere with the gene and the genetic engineering method to simulate the mutation. Romano Ibarra and others developed a megaTAL nuclease targeted at the third cell of CCR5 gene, which was transfected by mRNA.
【分类号】：R512.91
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本文编号：1749709