浙江省2004-2011年HIV-1流行毒株的传播和进化研究

发布时间：2018-04-19 06:15

本文选题：HIV-1 + 亚型　；参考：《浙江大学》2013年硕士论文

【摘要】：为了解浙江省HIV-1毒株的亚型分布、传播特征和流行趋势,我们在2004-2011年间募集部分HIV-1抗体阳性病例,采集静脉抗凝全血,提取前病毒DNA或病毒RNA,通过基因扩增、序列测定,获得HIV-1gag和pol基因片段。运用Mega等软件构建Neighbor-Joining系统进化树,分析基因亚型和遗传多样性;运用Simplot和RIP等软件分析新发重组毒株的重组断点；运用Bayesian系统进化分析并结合流行病学资料,探讨浙江省CRF01_AE毒株的起源、传播和流行趋势。研究表明,流行早期,HIV-1通过异性性接触途径传播进入浙江,进而播散流行,从高危人群向一般人群扩散；近几年,男男性行为人群中的HIV-1播散快速,成为浙江省最主要受累群体。浙江省内流行毒株亚型多样,来源复杂,主要毒株类型为CRF01_AE、CRF07_BC、CRF08_BC和B’(B),约占93%。但也存在C, G. CRF02_AG, CRF06_CPX等其他类型以及01B新发重组毒株(3个病例)和CRF07_BC/08_BC再重组毒株(5个病例),表明浙江HIV流行毒株类型复杂,疫情活跃,感染不同毒株的高危人群间存在交叉现象。这些特征符合浙江省艾滋病疫情多种途径、多种来源、以性接触传播为主的传播流行方式。从基因距离和Neighbor-Joining系统进化树判断,B亚型最早进入浙江,然后是B'、CRF01_AE、 CRF08_BC、CRF07_BC。Bayesian系统进化分析显示,CRF01_AE毒株1998年前后传播进入浙江,通过异性性接触和男男性行为进行广泛播散,并逐步成为浙江省的优势毒株。系统进化分析提示浙江与广西、江西和安徽等省病例具有直接或间接流行病学关联性。动态分析表明浙江省HIV疫情活跃,流行毒株复杂。浙江应加强与江西、安徽等周边省份联系,制定针对重点人群的预防和干预措施,提高防控效率,控制疫情蔓延。
[Abstract]:In order to understand the subtype distribution, transmission characteristics and epidemic trend of HIV-1 strains in Zhejiang Province, we collected some cases of HIV-1 antibody positive from 2004 to 2011, collected whole blood from vein anticoagulant, extracted DNA or viral RNAs from previrus, amplified them by gene amplification, and sequenced them.HIV-1gag and pol gene fragments were obtained.Neighbor-Joining phylogenetic tree was constructed by using Mega and other software to analyze gene subtypes and genetic diversity; Simplot and RIP were used to analyze the recombination breakpoints of new recombinant strains; Bayesian phylogenetic analysis and epidemiological data were used.To study the origin, transmission and epidemic trend of CRF01_AE strain in Zhejiang Province.Studies have shown that in the early stage of the epidemic, HIV-1 spread into Zhejiang through heterosexual contact and spread from high-risk groups to the general population. In recent years, HIV-1 has spread rapidly among men who have sex with men.To become the main affected groups in Zhejiang Province.In Zhejiang province, the subtypes of epidemic strains are various and the sources are complex. The main types of strains are CRF01- AEF07BCCO-CRF08-BC-BC0and BC0F08B, which account for 933% of the total number of strains in Zhejiang province.But there are other types such as C, GCRF02AG, CRF06_CPX, and new recombined strain 01B (3 cases) and recombination strain of CRF07_BC/08_BC (5 cases), which indicate that the prevalent strains of HIV in Zhejiang Province are complex in type and active in epidemic situation, and there is a cross phenomenon among the high risk population infected with different strains.These characteristics are consistent with the multiple ways and sources of AIDS epidemic in Zhejiang Province.Judging from the genetic distance and Neighbor-Joining phylogenetic tree, the subtype B first entered Zhejiang, and then it was BF01AE. CRF08BC07BC.Bayesian phylogenetic analysis showed that the strain of CRF01AE spread into Zhejiang province around 1998, and spread widely through heterosexual contact and male sex.And gradually become the dominant strain of Zhejiang Province.Phylogenetic analysis suggested that Zhejiang had direct or indirect epidemiological correlation with Guangxi, Jiangxi and Anhui provinces.Dynamic analysis showed that the epidemic situation of HIV in Zhejiang Province was active and the epidemic strains were complicated.Zhejiang should strengthen contact with neighboring provinces such as Jiangxi and Anhui, formulate prevention and intervention measures for key people, improve the efficiency of prevention and control, and control the spread of the epidemic.
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R512.91

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