基于磁性纳米探针的乙肝前S1抗原的快速磁性免疫层析方法的建立

发布时间：2018-04-21 04:42

本文选题：前S抗原 + 纳米磁珠　；参考：《生物学杂志》2016年01期

【摘要】：为了建立快速灵敏的乙肝磁性免疫层析检测方法,选用前S1抗原作为乙肝检测标志物,将前S1抗原的特异性单抗偶联到磁珠表面,制备了乙肝的特异性磁性纳米探针,并以此探针作为检测标记物制备了乙肝的磁性试纸条。采用乙肝临床血样,对所构建磁性试纸条的检测灵敏度、稳定性和重现性等检测性能进行了评价,并和市售的乙肝ELISA试剂盒进行了对比分析。与ELISA试剂盒的对比结果显示,研究所建立的乙肝磁性试纸条灵敏度较ELISA法高2倍,具有较好的稳定性(25℃可保存半年)和重现性;这两种方法对441份临床血样的检测结果一致性高达97%。研究可为今后乙肝的床边早期诊断产品开发提供技术支撑。
[Abstract]:In order to establish a rapid and sensitive detection method of hepatitis B magnetic immunochromatography, the pre S1 antigen was selected as the marker of hepatitis B detection, the specific monoclonal antibody of the former S1 antigen was coupled to the surface of the magnetic bead, and the specific magnetic nano probe of hepatitis B was prepared, and the magnetic test paper of hepatitis B was prepared by using this probe as a detection marker. The detection sensitivity, stability and reproducibility of the magnetic test paper were evaluated and compared with the commercial hepatitis B ELISA kit. Compared with the ELISA kit, the results showed that the sensitivity of the HBV magnetic test paper was 2 times higher than that of the ELISA method, and it had good stability (25 degrees C). It can be preserved for half a year) and reproducibility; the consistency of the two methods for the detection of 441 clinical blood samples up to 97%. can provide technical support for the development of the early diagnosis of hepatitis B by the hepatitis B.

【作者单位】：上海海洋大学食品学院上海水产品加工及贮藏工程技术研究中心;南京爱纳玛斯生物医药科技有限公司;同济大学医学院免疫与炎症研究中心;同济大学医学院生物医学工程与纳米科学研究院;
【基金】：国家自然科学基金(No.51173135) 上海市科委工程中心建设项目(11DZ2280300)
【分类号】：R446.6;R512.62

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