ERK抑制剂PD98059对泡状棘球蚴生长的影响

发布时间：2018-04-22 00:22

本文选题：泡状棘球蚴 + 原头节　；参考：《中国病原生物学杂志》2015年04期

【摘要】：目的探讨不同浓度ERK抑制剂PD98059体外对泡状棘球蚴原头节生长的作用及形态学影响。方法将体外培养的泡状棘球蚴分别加入6.25、12.5、25、50μmol/L PD98059中体外孵育,用0.1%伊红染色后在倒致显微镜下观察原头节形态并检测活力,试验重复3次;于透射电子显微镜(TEM)下观察PD98059作用后原头节超微结构变化;采用Caspase-3活性检测试剂盒检测原头节caspase-3酶活性。结果 DMEM/DMEM+DMSO培养组原头节活力较开始时无明显变化。50μmol/L PD98059作用48h后,原头节活力下降为(55.16±0.212)%,作用第14d,无存活的原头节。低浓度组对原头节的杀伤作用较高浓度组弱。TEM观察PD98059处理后的原头节微毛及糖萼出现部分缺损,合胞体带内出现少量小的空泡,且随用药浓度的增大,空泡增大增多并出现脂滴。ELISA检测显示,经PD98059处理的原头节caspase-3酶活性较正常对照组增强。结论 ERK抑制剂PD98059在体外有抗泡状棘球蚴作用。
[Abstract]:Objective to investigate the effects and morphological effects of different concentrations of ERK inhibitor PD98059 on the growth of echinococcosis in vitro. Methods the Echinococcus alveolar echinococcosis in vitro was incubated in 6.25,12.5,25,50 mu mol/L PD98059 respectively. The morphology of the original joint was observed under the inverted microscope and the activity was detected under the inverted microscope, and the experiment was repeated. 3 times; under the transmission electron microscope (TEM), the changes of the ultrastructure of the primary joint after PD98059 were observed. The Caspase-3 activity detection kit was used to detect the activity of the primary caspase-3 enzyme. Results there was no obvious change of.50 mu mol/L PD98059 in the DMEM/DMEM+DMSO culture group, and the activity of the original joint decreased to (55.16 + 0.212)%. 14d, no survival of the original joint. The low concentration group had a high concentration of KP in the lower concentration group and the weak.TEM observed the defects of the original head micro hair and the sugar calyx after PD98059 treatment, and a small number of small vacuoles appeared in the syncytial zone. With the increase of the concentration of the drugs, the increase of vacuoles increased and the lipid droplet.ELISA detection showed that PD98059 was found at PD98059. Conclusion caspase-3 inhibitor activity of the proto - crest is higher than that of the normal control group. Conclusion ERK inhibitor PD98059 has anti alveolar echinococcosis in vitro.

【作者单位】：石河子大学医学院组织胚胎学教研室;石河子大学第一附属医院肝胆外科;
【基金】：国家自然科学基金项目(No.81360410,U1303121)
【分类号】：R532.32

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