肝细胞癌合并乙型肝炎肝硬化患者癌组织中长链非编码RNA表达谱分析

发布时间：2018-04-25 14:10

本文选题：乙型肝炎 + 肝硬化　；参考：《郑州大学学报(医学版)》2015年02期

【摘要】：目的:观察长链非编码RNA(LncRNA)在肝细胞癌及癌旁肝硬化组织中表达谱的差异。方法:运用LncRNA表达谱芯片检测3例肝细胞癌组织及配对癌旁肝硬化组织中的LncRNA,对芯片检测的原始数据进行均一化处理和统计分析,采用分层聚类分析将2组间差异表达的LncRNA进行分类。从差异表达的LncRNA中选取4个LncRNA在10例中进行q PCR验证,以判断芯片检测结果的可靠性。结果:肝细胞癌组与癌旁肝硬化组2倍以上差异表达的LncRNA共2 225条,其中表达上调的747条(33.6%),表达下调的1 478条(66.4%),5倍以上表达上调的123条,5倍以上表达下调的353条。q PCR检测结果与芯片检测结果一致。结论:与癌旁肝硬化组织比较,肝细胞癌组织中LncRNA的表达谱具有显著变化。
[Abstract]:Aim: to observe the difference of LncRNAs expression profiles in hepatocellular carcinoma and paracancerous liver cirrhosis. Methods: LncRNA expression microarray was used to detect LncRNAs in 3 hepatocellular carcinoma tissues and matched liver cirrhosis tissues. The original data were homogenized and statistically analyzed. Stratified cluster analysis was used to classify the differentially expressed LncRNA between the two groups. Four LncRNA samples from differentially expressed LncRNA were selected for Q PCR verification in 10 cases to determine the reliability of the chip detection results. Results: there were 2 225 LncRNA differentially expressed in hepatocellular carcinoma group and paracancerous cirrhosis group. Among them, 747 up-regulated and 1 478 down-regulated 1 478 or 66.4 times more than 5 times of up-regulated expression were detected with 353.Q PCR. The results were consistent with the results of microarray detection. Conclusion: the expression profile of LncRNA in hepatocellular carcinoma is significantly different from that in paracancerous liver cirrhosis.
【作者单位】：深圳市第三人民医院病理科;
【基金】：深圳市科技创新计划项目JCYJ201404111137484100 深圳市科技计划项目(医疗卫生类)201202060
【分类号】：R735.7;R512.62;R575.2

【相似文献】