贵州省钩端螺旋体血清群特异性PCR鉴定结果与分析

发布时间：2018-04-26 16:11

本文选题：钩端螺旋体 + 血清群　；参考：《中国人兽共患病学报》2015年12期

【摘要】：目的采用血清群特异性PCR技术了解贵州省近年钩端螺旋体(钩体)流行菌群,为贵州省钩体病的防控提供技术手段和科学依据。方法采用致病性钩体特异性PCR方法(G1/G2-PCR)对来自贵州省近年的钩体分离菌株进行鉴定,进一步应用基于致病性钩体O抗原基因的血清群特异性PCR(O-PCR)对贵州省近年的58株钩体分离株进行血清群鉴定,并采用显微凝集试验(MAT)对O-PCR方法的检测结果进行验证。结果 G1/G2-PCR检测结果显示58株菌株均为致病性钩体,O-PCR将58株致病性钩体菌株鉴定为黄疸出血群,与传统的MAT法鉴定结果一致。结论 O-PCR技术可作为我省钩体快速分群鉴定可靠的实验室诊断技术手段,贵州省近年的钩体流行菌群为黄疸出血群。
[Abstract]:Objective to investigate the prevalence of leptospirosis (Leptospira) by serogroup specific PCR in Guizhou province in recent years so as to provide technical means and scientific basis for the prevention and control of leptospirosis in Guizhou. Methods the isolated strains of Leptospira isolated from Guizhou Province in recent years were identified by the method of pathogenic Leptospira specific PCR (G1 / G2-PCR). Furthermore, serogroup specific PCR- O-PCR based on pathogenic leptospirosis O antigen gene was used to identify 58 Leptospira isolates in Guizhou Province in recent years, and the results of O-PCR detection were verified by microagglutination test (MAT). Results the results of G1/G2-PCR analysis showed that 58 strains of pathogenic leptospirosis were identified as jaundice haemorrhage group by O-PCR, which was consistent with the traditional MAT method. Conclusion O-PCR technique can be used as a reliable laboratory diagnostic technique for rapid identification of Leptospira in Guizhou Province. In recent years, the endemic bacteria of Leptospira in Guizhou Province is jaundice haemorrhage group.
【作者单位】：贵州省疾病预防控制中心;贵州医科大学;贵阳市动物疫病预防控制中心;贵州大学动物科学院;
【基金】：国家自然科学基金项目(No.81260250,No.81401679) 贵州省传染病人才培养基地项目(黔人领发[2013]15)“自然疫源性和虫媒传染病研究团队(No.RCJD1401)” 贵州省疾病预防控制中心科学技术基金项目(No.2013-E2-1)联合资助~~
【分类号】：R514.4;R440

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