新疆维吾尔族及汉族乙型肝炎病毒感染与HLA-DP单核苷酸多态性的相关性研究

发布时间：2018-04-27 20:51

本文选题：慢性乙型肝炎病毒 + HLA-DP　；参考：《新疆医科大学》2014年硕士论文

【摘要】：目的：通过检测候选基因HLA-DP rs3077.rs2301220.rs3135021.rs9277341. rs9277378.rs9277542.rs9277535.rs9277471八个位点单核苷酸多态性,探讨新疆维吾尔族、汉族HLA-DP SNPs与慢性乙型肝炎病毒感染的相关性；探讨HLA-DP基因单核苷酸多态性是否影响维、汉两民族之间HBV感染的个体差异。为临床诊断、治疗及预后提供以分子学水平为指标的遗传学参考依据。方法：收集新疆医科大学中医医院、库尔勒维吾尔医院的住院和门诊患者以及体检人员作为受试者。所收样本分为维族、汉族慢性乙肝患者；维族、汉族健康者共四组,每组人数均等。采用1：1病例-对照研究,病例组为慢性乙肝患者,对照组为体检健康人群。1、使用DNA提取试剂盒提取标本基因组DNA并进行质检。2、用SNapshot方法对HLA-DP基因rs3077.rs2301220.rs3135021.rs9277341.rs9277378.rs9277542.rs9277535.rs9277471的八个位点进行检测及分型。3、统计分析采用SAS9.3。研究中所获得的数据,年龄采用均数和标准差表示,基因型频率和等位基因频率采用构成比或绝对数描述,先对维吾尔族与汉族之间进行Hardy-Weinberg平衡检验,乙肝组和非乙肝组之间基因多态性的差异采用χ2检验,分别以疾病、性别作为分层因素比较汉族和维吾尔族之间、乙肝非乙肝之间基因多态性的差异采用Cochran-Mantel-Haenselx2检验,不同基因型和有无乙肝之间的多因素分析采用Logistic回归,检验水准α=0.05,1-β=0.8。结果：1、在HBV+组和健康对照组比较中这三个位点的P值均小于0.05,具有统计学意义,故其位点多态性与HBV感染相关。2、在汉族HBV+与对照组比较时,个位点的P值均小于0.05,具有统计学意义。3、在汉族健康对照组与维吾尔族健康对照组比较,位点的单碱基分型具有统计学意义。4、汉族HBV+和维吾尔族HHBV+比较,rs3077这六个位点中单核苷酸多态性分型具有统计学意义。5、在性别与乙型肝炎病毒感染的相关性研究中发现健康对照组四个位点的单碱基分型是具有统计学意义的。结论：1、HLA-DP SNP与乙型肝炎病毒感染的关系, rs3077、rs2301220、rs9277341的三个位点与乙型肝炎病毒感染相关,其中rs3077GG、 rs2301220TT、rs9277341CC基因型可能增加乙型肝炎感染的风险。2、汉族健康对照组和维吾尔族健康对照组比较,发现rs3077、rs2301220、rs9277341、rs9277378、 rs9277542、rs9277471这六个基因位点具有统计学意义。其中rs3077AA、rs2301220CC、 rs9277341TT、rs9277378AA、rs9277542TT、rs9277471GG基因型有可能降低乙型肝炎病毒感染的可能性。3、男女性别差异与乙肝病毒感染的关系,rs9277341、rs9277378、 rs9277542、rs9277471四个位点可能与发病人群中的那女性别差异相关。rs9277341CC、 rs9277378GA、rs9277378GG、rs9277542CC基因型有可能增加乙型肝炎感染的风险。4、HLA-DP基因的基因频率是存在民族差异的,HLA-DP基因的rs3077、rs2301220、 rs9277341、rs9277378、rs9277542、rs9277535、rs9277471的七个位点中不同基因A、G、C、T的数目和基因频率在维吾尔族与汉族中存在显著差异,而rs3135021的两种等位基因A、G的数目和频率在两民族之间无统计学意义。
[Abstract]:Objective: To explore the correlation between the Xinjiang Uygur, the Han HLA-DP SNPs and the chronic hepatitis B virus infection by detecting the single nucleotide polymorphisms at the eight loci of the candidate gene HLA-DP rs3077.rs2301220.rs3135021.rs9277341. rs9277378.rs9277542.rs9277535.rs9277471, and to explore whether the single nucleotide polymorphism of the HLA-DP gene affects the dimension of the virus. The individual difference of HBV infection among the two ethnic groups. It provides a genetic reference basis for clinical diagnosis, treatment and prognosis. Methods: the hospital of traditional Chinese medicine of Xinjiang Medical University, the hospitalized and outpatient patients in Korla Uygur hospital and the medical staff were selected as subjects. The samples collected were divided into the Uygur ethnic group and the Han chronic B. The liver patients, the Uygur and the Han healthy people were four groups, each group was equal. The 1:1 case control study, the case group were chronic hepatitis B patients, the control group was a healthy population.1, the DNA extraction kit was used to extract the genomic DNA and the quality of the.2 was tested, and the SNapshot method was used for the HLA-DP gene rs3077.rs2301220.rs3135021.rs9277341.rs9277. The eight loci of 378.rs9277542.rs9277535.rs9277471 were detected and typed.3. Statistical analysis adopted the data obtained in the SAS9.3. study. The age was expressed by the average number and standard deviation. The genotype frequency and allele frequency were described by the composition ratio or the absolute number. The Hardy-Weinberg balance test was conducted between the Uygur and the Han nationality. The difference of gene polymorphism between the hepatitis B group and the non hepatitis B group was tested by the x 2 test. The difference of gene polymorphism between the Han and Uygur nationalities was compared with the sex as a stratified factor. The difference of gene polymorphism between hepatitis B and hepatitis B was detected by Cochran-Mantel-Haenselx2 test. The multiple factor analysis between the different genotypes and the hepatitis B and the hepatitis B was Logistic back. The results of alpha =0.05,1- beta =0.8. were as follows: 1, the P values of these three loci in the HBV+ group and the healthy control group were less than 0.05, so the polymorphism of the loci was associated with.2 in HBV infection. The P value of the loci was less than 0.05 in the Han HBV+ and the control group, with statistical significance.3, in the Han healthy control group and in the Han healthy control group. Compared with the Uygur healthy control group, the single base classification of the loci was statistically significant.4, the Han HBV+ and Uygur HHBV+ were compared, and the single nucleotide polymorphisms in the six loci of rs3077 had statistical significance.5. The single base fraction of the four sites in the healthy control group was found in the correlation between sex and hepatitis B virus infection. Conclusion: 1, 1, the relationship between HLA-DP SNP and HBV infection, the three loci of rs3077, rs2301220, rs9277341 are associated with hepatitis B virus infection, and rs3077GG, rs2301220TT, rs9277341CC genotype may increase the risk of hepatitis B infection in.2, Han healthy control group and Uygur health The six loci of rs3077, rs2301220, rs9277341, rs9277378, rs9277542, rs9277471 were statistically significant in the control group. Among them, rs3077AA, rs2301220CC, rs9277341TT, rs9277378AA, rs9277542TT, and the possibility of reducing the possibility of hepatitis B virus infection, sex differences between men and women and HBV infection The four loci of rs9277341, rs9277378, rs9277542, and rs9277471 may be related to.Rs9277341CC, rs9277378GA, rs9277378GG, and rs9277542CC genotypes that may increase the risk of hepatitis B infection, and the gene frequency of the HLA-DP gene is in ethnic differences, HLA-DP gene rs3077, and the rs3077 of HLA-DP gene. The number and frequency of A, G, C, T and gene frequency in the seven loci of rs9277341, rs9277378, rs9277542, rs9277535, and rs9277471 were significantly different between the Uygur and the Han people, while the two alleles of rs3135021 were A, and the number and frequency of G were not statistically significant between the two nationalities.

【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R512.62

【参考文献】